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are usually maintained in the 100–200 nM range (Bush, 1995; Webb et al., 1996) to prevent Ca2+ cytotoxicity by the formation of insoluble calcium precipitates with inorganic phosphates (Webb et al., 1996 ...
extract, sodium chloride, potassium chloride, calcium chloride, magnesium chloride, lithium chloride, and potassium hydroxide were purchased from Penta (Czech Republic). Magnesium nitrate hexahydrate ...
s on and 5.0 s off). The lysate was then centrifuged at 15 000 rpm for 15 min, and the protein was precipitated with a final concentration of 30% ammonium sulphate and incubated overnight at 4 °C ...
. Louis, MO) and stored at room temperature. Other chemicals used in the study were sodium chloride (VWR, West Chester, PA), potassium phosphate dibasic anhydrous, ammonium phosphate monobasic, magnesium ...
). Purified RNA samples were in 25 mM Tris–HCl, pH 8.0, 50 mM potassium chloride, 10 mM magnesium chloride, 0.5 mM cobalamin derivative. The observed gel mobility differences between samples were consistent ...
sulphate heptahydrate, potassium dichromate, sodium hydroxide, and nitric acid were purchased from Sigma Aldrich, and anhydrous calcium chloride, anhydrous disodium hydrogen phosphate , copper sulphate ...
and animals [1, 2]. P . mirabilis has also been identified as the causative agent of numerous human illnesses including cystitis, pyelonephritis, prostatitis, as well as intra-abdominal, wound, eye, and burn ...
. The SBF solution was prepared as previously described [36] by dissolving sodium chloride, potassium chloride, calcium chloride monohydrate (CaCl2.H2O), magnesium chloride hexahydrate (MgCl2.6H2O), sodium ...
, individual coordinates, three group B factors per residue and Translation Libration Screw-rotation (TLS) parameters were refined. Potential positions of magnesium or potassium ions were compared with those ...
(Gellissen et al.2005). SYN8 was rationally designed by exchange of the phosphate source and further ion reduction. This prevented the formation of precipitate and offered an electrical medium conductivity ...
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