The patch clamp technique was used to measure currents passing through K + channels in neuronal cell preparations. Retigabine (D-23129, N -(2-amino-4-(4-fluorobenzylamino)-phenyl) carbamic acid ethyl ester) activated a K + conductance in slightly depolarized NG108-15 neuronal cells in a dose dependent manner (0.1–10 μ M). At the K + reversal potential, no current could be elicited and in hyperpolarized cells the current was reversed. A similar current was elicited in primary cultures of mouse cortical neurones and in differentiated hNT cells, a cell line derived from human neuronal cells. At higher concentrations, retigabine also partially blocked voltage activated K + currents. None of the tested anticonvulsants, phenytoin, carbamazepine and valproate and none of the K + channel openers cromakalim, diazoxide and pinacidil exerted a similar effect. The current was not affected by the K + channel blocker glibenclamide (10 μ M) but was fully blocked by application of Ba 2+ (10.8 mM). Exchange of K + with cesium in the intracellular space also fully abolished the current. It can be expected that the K + channel opening effect contributes to the anticonvulsant activity of retigabine.
European Journal of Pharmacology – Elsevier
Published: Oct 8, 1997
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera