The Mouse SLIT Family: Secreted Ligands for ROBO Expressed in Patterns That Suggest a Role in Morphogenesis and Axon Guidance

The Mouse SLIT Family: Secreted Ligands for ROBO Expressed in Patterns That Suggest a Role in... The Slit gene encodes a secreted molecule essential for neural development in Drosophila embryos. Here we report the identification of three Slit homologues in the mouse. We demonstrate that the mouse SLIT1 protein can bind ROBO1, a transmembrane receptor implicated in axon guidance. Both whole-mount and section in situ hybridization studies reveal unique and complementary patterns of expression of the three mouse Slit genes and of Robo1, both within the central nervous system and in other developing tissues. The complementary expression patterns of Slit and Robo1 and their in vitro interaction suggest a ligand–receptor relationship. The expression of all three Slit genes in the floor plate suggests that they are likely to share the same functional properties with their Drosophila homologue in midline neural development and axon guidance. The complementary expression of Slit and Robo1 in different subdivisions of the somites suggests their possible function in axon pathfinding and neural crest cell migration. The unique expression pattern in limb and other organs indicates additional potential functions of the Slit gene family. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Biology Elsevier

The Mouse SLIT Family: Secreted Ligands for ROBO Expressed in Patterns That Suggest a Role in Morphogenesis and Axon Guidance

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Publisher
Elsevier
Copyright
Copyright © 1999 Academic Press
ISSN
0012-1606
eISSN
1095-564X
DOI
10.1006/dbio.1999.9371
Publisher site
See Article on Publisher Site

Abstract

The Slit gene encodes a secreted molecule essential for neural development in Drosophila embryos. Here we report the identification of three Slit homologues in the mouse. We demonstrate that the mouse SLIT1 protein can bind ROBO1, a transmembrane receptor implicated in axon guidance. Both whole-mount and section in situ hybridization studies reveal unique and complementary patterns of expression of the three mouse Slit genes and of Robo1, both within the central nervous system and in other developing tissues. The complementary expression patterns of Slit and Robo1 and their in vitro interaction suggest a ligand–receptor relationship. The expression of all three Slit genes in the floor plate suggests that they are likely to share the same functional properties with their Drosophila homologue in midline neural development and axon guidance. The complementary expression of Slit and Robo1 in different subdivisions of the somites suggests their possible function in axon pathfinding and neural crest cell migration. The unique expression pattern in limb and other organs indicates additional potential functions of the Slit gene family.

Journal

Developmental BiologyElsevier

Published: Aug 15, 1999

References

  • Role of the extracellular matrix in neural crest cell migration
    Henderson, D.J.; Copp, A.J.
  • Interactions of Eph-related receptors and ligands confer rostrocaudal pattern to trunk neural crest migration
    Krull, C.E.; Lansford, R.; Gale, N.W.; Collazo, A.; Marcelle, C.; Yancopoulos, G.D.; Fraser, S.E.; Bronner-Fraser, M.
  • Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening
    Nakayama, M.; Nakajima, D.; Nagase, T.; Nomura, N.; Seki, N.; Ohara, O.
  • Ectopic c-kit expression affects the fate of melanocyte precursors in Patch mutant embryos
    Wehrle-Haller, B.; Morrison-Graham, K.; Weston, J.A.

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