The influence of plant pyruvate, orthophosphate dikinase on a C 3 plant with respect to the intracellular location of the enzyme

The influence of plant pyruvate, orthophosphate dikinase on a C 3 plant with respect to the... We produced transgenic tobacco plants expressing the plastidic pyruvate, orthophosphate dikinase (PPDK) from the facultative Crassulacean acid metabolism plant Mesembryanthemum crystallinum . These transgenic plants had increased PPDK amounts in leaves and seeds. They produced more seeds per seed capsule and heavier seed capsules than the wildtype. When the enzyme was expressed in the cytosol by deleting the plastid signal sequence the transgenic tobacco plants produced less seeds than the wildtype and the transgenic line with the strongest phenotype produced nearly no seeds at all. All transgenic tobaccos were different than the wildtype with respect to the pattern of free amino acids in seeds and sink- and source leaves but not in roots. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Science Elsevier

The influence of plant pyruvate, orthophosphate dikinase on a C 3 plant with respect to the intracellular location of the enzyme

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Publisher
Elsevier
Copyright
Copyright © 1998 Elsevier Ltd
ISSN
0168-9452
D.O.I.
10.1016/S0168-9452(98)00090-9
Publisher site
See Article on Publisher Site

Abstract

We produced transgenic tobacco plants expressing the plastidic pyruvate, orthophosphate dikinase (PPDK) from the facultative Crassulacean acid metabolism plant Mesembryanthemum crystallinum . These transgenic plants had increased PPDK amounts in leaves and seeds. They produced more seeds per seed capsule and heavier seed capsules than the wildtype. When the enzyme was expressed in the cytosol by deleting the plastid signal sequence the transgenic tobacco plants produced less seeds than the wildtype and the transgenic line with the strongest phenotype produced nearly no seeds at all. All transgenic tobaccos were different than the wildtype with respect to the pattern of free amino acids in seeds and sink- and source leaves but not in roots.

Journal

Plant ScienceElsevier

Published: Aug 7, 1998

References

  • Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes
    Sheen, J.
  • Characterization of the gene for pyruvate, orthophosphate dikinase from rice, a C3 plant, and a comparison of structure and expression between C3 and C4 genes for this protein
    Imaizumi, N.; Ku, M.S.B.; Ishihara, K.; Samejima, M.; Kaneko, S.; Matsuoka, M.
  • Pyruvate orthophosphate dikinase mRNA organ specificity in wheat and maize
    Aoyagi, K.; Bassham, J.A.
  • Intracellular localization of some key enzymes of Crassulacean acid metabolism in Sedum praealtum
    Spalding, M.H.; Schmitt, M.R.; Ku, S.B.; Edwards, G.E.
  • Fructose-2,6-bisphosphate as a regulatory molecule in plants
    Stitt, M.
  • Pyruvate orthophosphate dikinase gene expression in developing wheat seeds
    Aoyagi, K.; Bassham, J.A.; Greene, F.C.
  • Lack of C 4 photosynthetic metabolism in ears of C 3 cereals
    Bort, J.; Brown, R.H.; Araus, J.L.

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