The Hippo Pathway Member Nf2 Is Required for Inner Cell Mass Specification

The Hippo Pathway Member Nf2 Is Required for Inner Cell Mass Specification Results and Discussion</h5> Nf2 Is Expressed in the Preimplantation Embryo</h5> Although multiple inputs have been proposed to regulate core Hippo signaling, the membrane-associated FERM domain protein neurofibromatosis 2 (Nf2, the ortholog of Drosophila Merlin) remains the only upstream component shown to be required for Hippo signaling across a broad range of cell types [ 2–6 ] and to be genetically required for Hippo signaling in vivo [ 6, 7 ]. Given its requirement for Hippo signaling in other cell types, we reasoned that Nf2 might also play a role upstream of the Hippo pathway kinases Lats 1 and 2 (Lats1/2) and Yap in the segregation of trophectoderm (TE) and inner cell mass (ICM). To determine whether this could be the case, we first investigated its expression profile during preimplantation development. We performed qPCR on pools of embryos at nine different preimplantation stages and found that, while it could be detected throughout preimplantation development, Nf2 mRNA was especially abundant prior to the two-cell stage, suggesting that it is maternally deposited ( Figure 1 A). To examine the localization of Nf2 protein in the preimplantation embryo, we cultured embryos that had been injected with GFP-tagged Nf2 mRNA to the morula http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Biology Elsevier

The Hippo Pathway Member Nf2 Is Required for Inner Cell Mass Specification

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Publisher
Elsevier
Copyright
Copyright © 2013 Elsevier Ltd
ISSN
0960-9822
D.O.I.
10.1016/j.cub.2013.05.044
Publisher site
See Article on Publisher Site

Abstract

Results and Discussion</h5> Nf2 Is Expressed in the Preimplantation Embryo</h5> Although multiple inputs have been proposed to regulate core Hippo signaling, the membrane-associated FERM domain protein neurofibromatosis 2 (Nf2, the ortholog of Drosophila Merlin) remains the only upstream component shown to be required for Hippo signaling across a broad range of cell types [ 2–6 ] and to be genetically required for Hippo signaling in vivo [ 6, 7 ]. Given its requirement for Hippo signaling in other cell types, we reasoned that Nf2 might also play a role upstream of the Hippo pathway kinases Lats 1 and 2 (Lats1/2) and Yap in the segregation of trophectoderm (TE) and inner cell mass (ICM). To determine whether this could be the case, we first investigated its expression profile during preimplantation development. We performed qPCR on pools of embryos at nine different preimplantation stages and found that, while it could be detected throughout preimplantation development, Nf2 mRNA was especially abundant prior to the two-cell stage, suggesting that it is maternally deposited ( Figure 1 A). To examine the localization of Nf2 protein in the preimplantation embryo, we cultured embryos that had been injected with GFP-tagged Nf2 mRNA to the morula

Journal

Current BiologyElsevier

Published: Jul 8, 2013

References

  • YAP1 is involved in mesothelioma development and negatively regulated by Merlin through phosphorylation
    Yokoyama, T.; Osada, H.; Murakami, H.; Tatematsu, Y.; Taniguchi, T.; Kondo, Y.; Yatabe, Y.; Hasegawa, Y.; Shimokata, K.; Horio, Y.
  • Structural analysis of Drosophila merlin reveals functional domains important for growth control and subcellular localization
    LaJeunesse, D.R.; McCartney, B.M.; Fehon, R.G.
  • Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo
    Ralston, A.; Rossant, J.

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