Results and Discussion</h5> Nf2 Is Expressed in the Preimplantation Embryo</h5> Although multiple inputs have been proposed to regulate core Hippo signaling, the membrane-associated FERM domain protein neurofibromatosis 2 (Nf2, the ortholog of Drosophila Merlin) remains the only upstream component shown to be required for Hippo signaling across a broad range of cell types [ 2–6 ] and to be genetically required for Hippo signaling in vivo [ 6, 7 ]. Given its requirement for Hippo signaling in other cell types, we reasoned that Nf2 might also play a role upstream of the Hippo pathway kinases Lats 1 and 2 (Lats1/2) and Yap in the segregation of trophectoderm (TE) and inner cell mass (ICM). To determine whether this could be the case, we first investigated its expression profile during preimplantation development. We performed qPCR on pools of embryos at nine different preimplantation stages and found that, while it could be detected throughout preimplantation development, Nf2 mRNA was especially abundant prior to the two-cell stage, suggesting that it is maternally deposited ( Figure 1 A). To examine the localization of Nf2 protein in the preimplantation embryo, we cultured embryos that had been injected with GFP-tagged Nf2 mRNA to the morula
Current Biology – Elsevier
Published: Jul 8, 2013
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera