The endovanilloid/endocannabinoid system in human osteoclasts: Possible involvement in bone formation and resorption

The endovanilloid/endocannabinoid system in human osteoclasts: Possible involvement in bone... Recent studies suggest a role for the endocannabinoid/endovanilloid anandamide in the regulation of bone resorption/formation balance in mice. Here, we examined the co-expression of the transient receptor potential vanilloid type 1 (TRPV1) and the cannabinoid CB1/CB2 receptors together with N-acylphosphatidylethanolamine-hydrolizing phospholipase D (NAPE-PLD) and fatty acid amide hydrolase (FAAH), the two enzymes responsible of the synthesis and catabolism of anandamide respectively, in human osteoclasts. Co-expression of TRPV1, CB1/CB2, NAPE-PLD and FAAH was found in both human osteoclast cultures and in native osteoclasts from human bone biopsies. Moreover, agonist-evoked calcium entry indicated that the TRPV1 receptor is functionally active in vitro. Consistently, biomolecular and functional experiments showed that resiniferatoxin (RTX), a selective TRPV1 receptor agonist, increased the expression and the activity of TRAP and cathepsin K, two specific osteoclast biomarkers. The evidence that cannabinoid and vanilloid receptors are co-expressed in human osteoclasts suggests that they might cross-talk to modulate the intrinsic balance of bone mineralization and resorption by different actions of anandamide through TRPV1 and cannabinoid receptors. The presence of the endocannabinoid/endovanilloid proteins in human osteoclasts will likely have implications for the management of bone demineralization associated syndrome (i. e. osteoporosis). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Bone Elsevier

The endovanilloid/endocannabinoid system in human osteoclasts: Possible involvement in bone formation and resorption

Loading next page...
 
/lp/elsevier/the-endovanilloid-endocannabinoid-system-in-human-osteoclasts-possible-b0cMMrr0N2
Publisher
Elsevier
Copyright
Copyright © 2008 Elsevier Inc.
ISSN
8756-3282
D.O.I.
10.1016/j.bone.2008.10.056
Publisher site
See Article on Publisher Site

Abstract

Recent studies suggest a role for the endocannabinoid/endovanilloid anandamide in the regulation of bone resorption/formation balance in mice. Here, we examined the co-expression of the transient receptor potential vanilloid type 1 (TRPV1) and the cannabinoid CB1/CB2 receptors together with N-acylphosphatidylethanolamine-hydrolizing phospholipase D (NAPE-PLD) and fatty acid amide hydrolase (FAAH), the two enzymes responsible of the synthesis and catabolism of anandamide respectively, in human osteoclasts. Co-expression of TRPV1, CB1/CB2, NAPE-PLD and FAAH was found in both human osteoclast cultures and in native osteoclasts from human bone biopsies. Moreover, agonist-evoked calcium entry indicated that the TRPV1 receptor is functionally active in vitro. Consistently, biomolecular and functional experiments showed that resiniferatoxin (RTX), a selective TRPV1 receptor agonist, increased the expression and the activity of TRAP and cathepsin K, two specific osteoclast biomarkers. The evidence that cannabinoid and vanilloid receptors are co-expressed in human osteoclasts suggests that they might cross-talk to modulate the intrinsic balance of bone mineralization and resorption by different actions of anandamide through TRPV1 and cannabinoid receptors. The presence of the endocannabinoid/endovanilloid proteins in human osteoclasts will likely have implications for the management of bone demineralization associated syndrome (i. e. osteoporosis).

Journal

BoneElsevier

Published: Mar 1, 2009

References

  • Cannabinoid receptors and the regulation of bone mass
    Bab, I.; Zimmer, A.
  • The endogenous lipid anandamide is a full agonist at the human vanilloid receptor (hVR1)
    Smart, D.; Gunthorpe, M.J.; Jerman, J.C.; Nasir, S.; Gray, J.; Muir, A.I.
  • Cellular accumulation of anandamide: consensus and controversy
    Hillard, C.; Jarrahian, A.
  • Anandamide regulates neuropeptide release from capsaicin-sensitive primary sensory neurons by activating both the cannabinoid 1 receptor and the vanilloid receptor 1 in vitro
    Ahluwalia, J.; Urban, L.; Bevan, S.; Nagy, I.
  • Suppression of fibroblast metalloproteinases by ajulemic acid, a nonpsychoactive cannabinoid acid
    Johnson, D.R.; Stebulis, J.A.; Rossetti, R.G.; Burstein, S.H.; Zurier, R.B.
  • Transient receptor potential cation channels in disease
    Nilius, B.; Owsianik, G.; Voets, T.; Peters, J.A.
  • Osteoclasts play a part in pain due to the inflammation adjacent to bone
    Nagae, M.; Hiraga, T.; Wakabayashi, H.; Wang, L.; Iwata, K.; Yoneda, T.
  • Endocannabinoids and the regulation of bone metabolism
    Bab, I.; Ofek, O.; Tam, J.; Rehnelt, J.; Zimmer, A.
  • Cannabinoid receptor type 2 gene is associated with human osteoporosis
    Karsak, M.; Cohen-Solal, M.; Freudenberg, J.; Ostertag, A.; Morieux, C.; Kornak, U.
  • Analgesic actions of N-arachidonoyl-serotonin, a fatty acid amide hydrolase inhibitor with antagonistic activity at vanilloid TRPV1 receptors
    Maione, S.; De Petrocellis, L.; de Novellis, V.; Moriello, A.S.; Petrosino, S.; Palazzo, E.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off