The detection of small round-structured viruses in water and environmental materials

The detection of small round-structured viruses in water and environmental materials A procedure for concentrating small round-structured viruses (SRSVs) (Norwalk-like viruses) from water and other environmental materials is described. Primers based on the helicase region of the SRSV genome were confirmed as specific by reaction with typed specimens, and used to detect virus in concentrates of unseeded and seeded samples. Virus was detected in estuarine recreational water polluted by untreated sewage, although not in seawater samples taken some distance from outfall discharges. It was also detected in river water downstream of a sewage treatment plant. Virus could be detected in all matrices when they were seeded with a positive stool extract, including sewage seeded with as little as 2 μl stool extract, thus confirming the suitability of the method for environmental monitoring. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

The detection of small round-structured viruses in water and environmental materials

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Publisher
Elsevier
Copyright
Copyright © 2000 Elsevier Science B.V.
ISSN
0166-0934
eISSN
1879-0984
DOI
10.1016/S0166-0934(00)00157-9
Publisher site
See Article on Publisher Site

Abstract

A procedure for concentrating small round-structured viruses (SRSVs) (Norwalk-like viruses) from water and other environmental materials is described. Primers based on the helicase region of the SRSV genome were confirmed as specific by reaction with typed specimens, and used to detect virus in concentrates of unseeded and seeded samples. Virus was detected in estuarine recreational water polluted by untreated sewage, although not in seawater samples taken some distance from outfall discharges. It was also detected in river water downstream of a sewage treatment plant. Virus could be detected in all matrices when they were seeded with a positive stool extract, including sewage seeded with as little as 2 μl stool extract, thus confirming the suitability of the method for environmental monitoring.

Journal

Journal of Virological MethodsElsevier

Published: Jun 1, 2000

References

  • Sequence similarity of human caliciviruses and small round structured viruses
    Cubitt, W.D.; Jiang, X.J.; Wang, J.; Estes, M.K.
  • Norwalk-like gastroenteritis in a Toronto hospital
    Leers, W.; Kasupski, G.; Fralick, R.; Whatman, S.; Garcia, J.; Gray, W.

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