The concept of calcium concentration microdomains in synaptic transmission

The concept of calcium concentration microdomains in synaptic transmission Ever since the initial measurements of presynaptic calcium currents it has been evident that calcium triggers transmitter release quite rapidly. Several models indicate, as did the initial voltage clamp measurements, that the calcium concentration triggering such release could be very high at the entry site and that this concentration should be very short lasting. In order to determine this time course, calcium entry was studied at the squid giant synapse by imaging light emission from n -aequorin-J, intracellularly injected into the presynaptic terminal. The imaging utilized a video system capable of acquiring 4000 frames per sec. The results indicate that the calcium entry, triggered by action potentials, reaches a peak within 200 μsec and has an overall duration of close to 800 μsec, closely matching the duration of the presynaptic calcium current determined by voltage clamp results under similar conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuropharmacology Elsevier

The concept of calcium concentration microdomains in synaptic transmission

Neuropharmacology, Volume 34 (11) – Nov 1, 1995

Loading next page...
 
/lp/elsevier/the-concept-of-calcium-concentration-microdomains-in-synaptic-migt90Jo91
Publisher
Elsevier
Copyright
Copyright © 1995 Elsevier Ltd
ISSN
0028-3908
eISSN
1873-7064
D.O.I.
10.1016/0028-3908(95)00150-5
Publisher site
See Article on Publisher Site

Abstract

Ever since the initial measurements of presynaptic calcium currents it has been evident that calcium triggers transmitter release quite rapidly. Several models indicate, as did the initial voltage clamp measurements, that the calcium concentration triggering such release could be very high at the entry site and that this concentration should be very short lasting. In order to determine this time course, calcium entry was studied at the squid giant synapse by imaging light emission from n -aequorin-J, intracellularly injected into the presynaptic terminal. The imaging utilized a video system capable of acquiring 4000 frames per sec. The results indicate that the calcium entry, triggered by action potentials, reaches a peak within 200 μsec and has an overall duration of close to 800 μsec, closely matching the duration of the presynaptic calcium current determined by voltage clamp results under similar conditions.

Journal

NeuropharmacologyElsevier

Published: Nov 1, 1995

References

  • Calcium entry into voltage-clamped presynaptic terminals of squid
    Augustine, G.J.; Charlton, M.P.; Smith, S.J.
  • Role of presynaptic calcium ions and channels in synaptic facilitation and depression at the squid giant synapse
    Charlton, M.P.; Smith, S.J.; Zucker, R.S.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off