Available online at www.sciencedirect.com ScienceDirect Synthetic biology — application-oriented cell engineering 1 1 1,2 Mingqi Xie , Viktor Haellman and Martin Fussenegger Synthetic biology applies engineering principles to biological their extension to more diverse applications or speciﬁca- systems and reprograms living cells to perform novel and tions [3,4]. improved functions. In this review, we ﬁrst provide an update of common tools and design principles that enable user- Cell engineering tools deﬁned control of mammalian cell activities with Genome editing spatiotemporal precision. Next, we demonstrate some As the natural ability of cells to control their behaviour is examples of how engineered mammalian cells can be encoded at their genetic level, the capability to engineer developed towards biomedical solutions in the context of complex cell functionalities directly relates to our ex- real-world problems. pertise in manipulating endogenous gene networks. In Addresses recent years, the emergent CRISPR/Cas9 technology Department of Biosystems Science and Engineering, ETH Zurich, enabling facile and permanent modiﬁcation of genomic Mattenstrasse 26, CH-4058 Basel, Switzerland sequences in mammalian cells and animals has brought University of Basel, Faculty of Science, Mattenstrasse 26, CH-4058 the promise of gene editing designer nucleases back Basel, Switzerland into the limelight . Classical designer nucleases,
Current Opinion in Biotechnology – Elsevier
Published: Aug 1, 2016
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