•SYBR Green I-based real-time RT-PCR in combination with melting curve analysis (MCA) was optimized for the detection and discrimination of grapevine viruses.•The assay was evaluated to confirm the feasibility of this technique in the detection of grapevine viruses from field samples and insect vectors.•Besides virus diagnostics, the SYBR Green I-based real-time RT-PCR MCA assay provides a superior resolution power to examine competition dynamics among frequently co-occurring viruses in grapevines and explore the consequences of co-infections on virus-host interactions and spread of individual viruses.
Journal of Virological Methods – Elsevier
Published: Sep 1, 2016
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud