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du Québec (CIAQ Inc., St- Hyacinthe , QC, Canada). For each experiment, straws containing frozen pooled semen from five bulls were thawed in a water bath at 37°C for 1 min. The semen was washed twice ...
(DABCO), deoxyribonuclease I (DNAse I), Hoechst 33342, protease inhibitors and mouse, rabbit and goat IgGs that were used as controls were obtained from Sigma-Aldrich (St Louis, MO, USA). VECTASHIELD ...
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