Suppression of cytotoxin-induced cell death in isolated hepatocytes by tea catechins

Suppression of cytotoxin-induced cell death in isolated hepatocytes by tea catechins To elucidate the hepatoprotective effects of green tea catechins, the following experiments were conducted utilizing (−)-epigallocatechin-3-gallate (EGCG), the major component of green tea catechin, together with other catechins. The protective effects of catechins against hepatotoxins, bromobenzene or rubratoxin B, were examined in primary cultures of rat hepatocytes. Bromobenzene and rubratoxin B are known to induce necrosis and apoptosis of cells, respectively. After 24-h treatment with toxin, EGCG and (−)-epigallocatechin-3-(3″- O -methyl)gallate (EGCg-3″-OMe) suppressed the bromobenzene-induced morphological change and dose-dependently prevented bromobenzene-induced cell death. Both catechins also prevented apoptotic cell death caused by rubratoxin B. In rubratoxin B-treated cells, both catechins were found to suppress the activation of caspase-3 by rubratoxin B. The results in the present study suggest that EGCG and EGCg-3″-OMe are potent hepatoprotective agents. This report is the first to show that catechins suppress cytotoxin-induced cell death. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Pharmacology Elsevier

Suppression of cytotoxin-induced cell death in isolated hepatocytes by tea catechins

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science B.V.
ISSN
0014-2999
DOI
10.1016/S0014-2999(02)02157-X
Publisher site
See Article on Publisher Site

Abstract

To elucidate the hepatoprotective effects of green tea catechins, the following experiments were conducted utilizing (−)-epigallocatechin-3-gallate (EGCG), the major component of green tea catechin, together with other catechins. The protective effects of catechins against hepatotoxins, bromobenzene or rubratoxin B, were examined in primary cultures of rat hepatocytes. Bromobenzene and rubratoxin B are known to induce necrosis and apoptosis of cells, respectively. After 24-h treatment with toxin, EGCG and (−)-epigallocatechin-3-(3″- O -methyl)gallate (EGCg-3″-OMe) suppressed the bromobenzene-induced morphological change and dose-dependently prevented bromobenzene-induced cell death. Both catechins also prevented apoptotic cell death caused by rubratoxin B. In rubratoxin B-treated cells, both catechins were found to suppress the activation of caspase-3 by rubratoxin B. The results in the present study suggest that EGCG and EGCg-3″-OMe are potent hepatoprotective agents. This report is the first to show that catechins suppress cytotoxin-induced cell death.

Journal

European Journal of PharmacologyElsevier

Published: Aug 30, 2002

References

  • Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells
    Islam, S.; Islam, N.; Kermode, T.; Johnstone, B.; Mukhtar, H.; Moskowitz, R.W.; Goldberg, V.M.; Malemud, C.J.; Haqqi, T.M.
  • Apoptosis induced by tea polyphenols in HL-60 cells
    Zhao, Y.; Cao, J.; Ma, H.; Liu, J.

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