Loading next page...
/lp/elsevier/subcellular-localization-of-interacting-proteins-by-bimolecular-x0ASSdqYL5
- Publisher
- Elsevier
- Copyright
- Copyright © 2006 Elsevier Ltd
- ISSN
- 0022-2836
- DOI
- 10.1016/j.jmb.2006.08.017
- Publisher site
- See Article on Publisher Site
Abstract
Bimolecular fluorescence complementation (BiFC) represents one of the most advanced and powerful tools for studying and visualizing protein–protein interactions in living cells. In this method, putative interacting protein partners are fused to complementary non-fluorescent fragments of an autofluorescent protein, such as the yellow spectral variant of the green fluorescent protein. Interaction of the test proteins may result in reconstruction of fluorescence if the two portions of yellow spectral variant of the green fluorescent protein are brought together in such a way that they can fold properly. BiFC provides an assay for detection of protein–protein interactions, and for the subcellular localization of the interacting protein partners. To facilitate the application of BiFC to plant research, we designed a series of vectors for easy construction of N-terminal and C-terminal fusions of the target protein to the yellow spectral variant of the green fluorescent protein fragments. These vectors carry constitutive expression cassettes with an expanded multi-cloning site. In addition, these vectors facilitate the assembly of BiFC expression cassettes into Agrobacterium multi-gene expression binary plasmids for co-expression of interacting partners and additional autofluorescent proteins that may serve as internal transformation controls and markers of subcellular compartments. We demonstrate the utility of these vectors for the analysis of specific protein–protein interactions in various cellular compartments, including the nucleus, plasmodesmata, and chloroplasts of different plant species and cell types.
Journal
Journal of Molecular Biology – Elsevier
Published: Oct 6, 2006
Recommended Articles
Loading...
References
-
Regulating access to the genome. Nucleocytoplasmic transport throughout the cell cycleWeis, K.
-
Anything else but GAGA: a nonhistone protein complex reshapes chromatin structureLehmann, M.
-
Eukaryotic transcriptional regulatory complexes: cooperativity from near and afarOgata, K.; Sato, K.; Tahirov, T.H.; Tahirov, T.
-
Partners-in-infection: host proteins involved in the transformation of plant cells by AgrobacteriumTzfira, T.; Citovsky, V.
-
Cytokinin signal perception and transductionHeyl, A.; Schmulling, T.
-
Transcription factors do it together: the hows and whys of studying protein-protein interactionsImmink, R.G.; Angenent, G.C.
-
Protein-protein interactions: methods for detection and analysisPhizicky, E.M.; Fields, S.
-
Interaction of myogenic factors and the retinoblastoma protein mediates muscle cell commitment and differentiationGu, W.; Schneider, J.W.; Condorelli, G.; Kaushal, S.; Mahdavi, V.; Nadal-Ginard, B.
-
Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoDHalevy, O.; Novitch, B.G.; Spicer, D.B.; Skapek, S.X.; Rhee, J.; Hannon, G.J.
-
A novel genetic system to detect protein-protein interactionsFields, S.; Song, O.-K.
-
Analysing protein-protein interactions with the yeast two-hybrid systemCausier, B.; Davies, B.
-
Genome-wide protein interaction screens reveal functional networks involving Sm-like proteinsFromont-Racine, M.; Mayes, A.E.; Brunet-Simon, A.; Rain, J.C.; Colley, A.; Dix, I.
-
The yeast two-hybrid system: criteria for detecting physiologically significant protein-protein interactionsGolemis, E.A.; Serebriiskii, I.; Law, S.F.
-
Isolation of an AP-1 repressor by a novel method for detecting protein- protein interactionsAronheim, A.; Zandi, E.; Hennemann, H.; Elledge, S.J.; Karin, M.
-
A novel in vivo assay for the analysis of protein-protein interactionMaroun, M.; Aronheim, A.
-
The Ras recruitment system, a novel approach to the study of protein–protein interactionsBroder, Y.C.; Katz, S.; Aronheim, A.
-
Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleusDeslandes, L.; Olivier, J.; Peeters, N.; Feng, D.X.; Khounlotham, M.; Boucher, C.
-
High-throughput two-hybrid analysis. The promise and the perilFields, S.
-
Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementationRossi, F.; Charlton, C.A.; Blau, H.M.
-
Monitoring protein-protein interactions in live mammalian cells by beta-galactosidase complementationRossi, F.M.; Blakely, B.T.; Charlton, C.A.; Blau, H.M.
-
Gene expression and cell fusion analyzed by lacZ complementation in mammalian cellsMohler, W.A.; Blau, H.M.
-
Direct visualization of protein interactions in plant cellsSubramaniam, R.; Desveaux, D.; Spickler, C.; Michnick, S.W.; Brisson, N.
-
Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizationsSekar, R.B.; Periasamy, A.
-
Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleusDay, R.N.; Periasamy, A.; Schaufele, F.
-
Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDIDel Pozo, M.A.; Kiosses, W.B.; Alderson, N.B.; Meller, N.; Hahn, K.M.; Schwartz, M.A.
-
Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cellElangovan, M.; Day, R.N.; Periasamy, A.
-
Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopyElangovan, M.; Wallrabe, H.; Chen, Y.; Day, R.N.; Barroso, M.; Periasamy, A.
-
Effects of calreticulin on viral cell-to-cell movementChen, M.H.; Tian, G.W.; Gafni, Y.; Citovsky, V.
-
The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methodsBhat, R.A.; Lahaye, T.; Panstruga, R.
-
Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementationHu, C.D.; Chinenov, Y.; Kerppola, T.K.
-
Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysisHu, C.D.; Kerppola, T.K.
-
Visualization of G protein beta gamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targetingHynes, T.R.; Tang, L.; Mervine, S.M.; Sabo, J.L.; Yost, E.A.; Devreotes, P.N.; Berlot, C.H.
-
Visualization of Myc/Max/Mad family dimers and the competition for dimerization in living cellsGrinberg, A.V.; Hu, C.D.; Kerppola, T.K.
-
VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciensAtmakuri, K.; Ding, Z.; Christie, P.J.
-
Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene familyTsuchisaka, A.; Theologis, A.
-
The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformationLacroix, B.; Vaidya, M.; Tzfira, T.; Citovsky, V.
-
Involvement of targeted proteolysis in plant genetic transformation by AgrobacteriumTzfira, T.; Vaidya, M.; Citovsky, V.
-
Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by AgrobacteriumLi, J.; Krichevsky, A.; Vaidya, M.; Tzfira, T.; Citovsky, V.
-
The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of endosperm-specific genes during barley seed developmentDiaz, I.; Martinez, M.; Isabel-LaMoneda, I.; Rubio-Somoza, I.; Carbonero, P.
-
In planta analysis of protein-protein interactions related to light signaling by bimolecular fluorescence complementationStolpe, T.; Susslin, C.; Marrocco, K.; Nick, P.; Kretsch, T.; Kircher, S.
-
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementationWalter, M.; Chaban, C.; Schütze, K.; Batistic, O.; Weckermann, K.; Näke, C.
-
Detection of protein-protein interactions in plants using bimolecular fluorescence complementationBracha-Drori, K.; Shichrur, K.; Katz, A.; Oliva, M.; Angelovici, R.; Yalovsky, S.; Ohad, N.
-
FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apexAbe, M.; Kobayashi, Y.; Yamamoto, S.; Daimon, Y.; Yamaguchi, A.; Ikeda, Y.
-
pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plantsTzfira, T.; Tian, G.-W.; Lacroix, B.; Vyas, S.; Li, J.; Leitner-Dagan, Y.
-
A versatile vector system for multiple gene expression in plantsChung, S.M.; Frankman, E.L.; Tzfira, T.
-
Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent proteinYang, T.T.; Cheng, L.; Kain, S.R.
-
Cooperative interaction of Agrobacterium VirE2 protein with single stranded DNA: implications for the T-DNA transfer processCitovsky, V.; Wong, M.L.; Zambryski, P.C.
-
Mutagenesis of the Agrobacterium VirE2 single-stranded DNA-binding protein identifies regions required for self-association and interaction with VirE1 and a permissive site for hybrid protein constructionZhou, X.R.; Christie, P.J.
-
Functional domains of Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2Dombek, P.; Ream, L.W.
-
Genetically modified plants and the 35S promoter: assessing the risk and enhancing the debateHull, R.; Covey, S.N.; Dale, P.
-
Nuclear transport of plant potyviral proteinsRestrepo, M.A.; Freed, D.D.; Carrington, J.C.
-
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinShaner, N.C.; Campbell, R.E.; Steinbach, P.A.; Giepmans, B.N.; Palmer, A.E.; Tsien, R.Y.
-
The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cellsHoward, E.A.; Zupan, J.R.; Citovsky, V.; Zambryski, P.C.
-
Genetic analysis of the virD operon of Agrobacterium tumefaciens : a search for functions involved in transport of T-DNA into the plant cell nucleus and in T-DNA integrationKoukolikova-Nicola, Z.; Raineri, D.; Stephens, K.; Ramos, C.; Tinland, B.; Nester, E.W.; Hohn, B.
-
Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of AgrobacteriumGuralnick, B.; Thomsen, G.; Citovsky, V.
-
Role of the Agrobacterium tumefaciens VirD2 protein in T-DNA transfer and integrationMysore, K.S.; Bassuner, B.; Deng, X.-B.; Darbinian, N.S.; Motchoulski, A.; Ream, L.W.; Gelvin, S.B.
-
Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 proteinBallas, N.; Citovsky, V.
-
Plasmodesmata as a supracellular control network in plantsLucas, W.J.; Lee, J.Y.
-
Sink plasmodesmata as gateways for phloem unloading. Myosin VIII and calreticulin as molecular determinants of sink strength?Baluska, F.; Cvrckova, F.; Kendrick-Jones, J.; Volkmann, D.
-
Maize calreticulin localizes preferentially to plasmodesmata in root apexBaluska, F.; Samaj, J.; Napier, R.; Volkmann, D.
-
Secretory pathway and the cytoskeleton in intracellular targeting and tubule assembly of Grapevine fanleaf virus movement protein in tobacco BY-2 cellsLaporte, C.; Vetter, G.; Loudes, A.M.; Robinson, D.G.; Hillmer, S.; Stussi-Garaud, C.; Ritzenthaler, C.
-
Gating of epidermal plasmodesmata is restricted to the leading edge of expanding infection sites of tobacco mosaic virus (TMV)Oparka, K.J.; Prior, D.A.M.; Santa-Cruz, S.; Padgett, H.S.; Beachy, R.N.
-
Isolation and regulation of accumulation of a minor chromoplast-specific protein from cucumber corollasLibal-Weksler, Y.; Vishnevetsky, M.; Ovadis, M.; Vainstein, A.
-
A set of modular plant transformation vectors allowing flexible insertion of up to six expression unitsGoderis, I.J.; De Bolle, M.F.; Francois, I.E.; Wouters, P.F.; Broekaert, W.F.; Cammue, B.P.
-
Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cellsKunik, T.; Palanichelvam, K.; Czosnek, H.; Citovsky, V.; Gafni, Y.
-
Tomato yellow leaf curl virus (TYLCV) capsid protein (CP) subunit interactions: implications for viral assemblyHallan, V.; Gafni, Y.
-
Increase in plasmodesmatal permeability during cell-to-cell spread of tobacco rattle tobravirus from individually inoculated cellsDerrick, P.M.; Barker, H.; Oparka, K.J.
-
Tobacco mosaic virus movement protein-mediated protein transport between trichome cellsWaigmann, E.; Zambryski, P.
-
Subcellular distribution of mutant movement proteins of Cucumber mosaic virus fused to green fluorescent proteinsCanto, T.; Palukaitis, P.
-
Identification of regions of the Beet mild curly top virus (family Geminiviridae) capsid protein involved in systemic infection, virion formation and leafhopper transmissionSoto, M.J.; Chen, L.F.; Seo, Y.S.; Gilbertson, R.L.
-
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alphaConti, E.; Uy, M.; Leighton, L.; Blobel, G.; Kuriyan, J.
-
Confocal optics microscopy for biochemical and cellular high-throughput screeningZemanova, L.; Schenk, A.; Valler, M.J.; Nienhaus, G.U.; Heilker, R.
-
The power and prospects of fluorescence microscopies and spectroscopiesMichalet, X.; Kapanidis, A.N.; Laurence, T.; Pinaud, F.; Doose, S.; Pflughoefft, M.; Weiss, S.
-
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisNorrander, J.; Kempe, T.; Messing, J.
-
The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding proteinCitovsky, V.; Knorr, D.; Schuster, G.; Zambryski, P.C.
-
VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivityTzfira, T.; Vaidya, M.; Citovsky, V.
-
Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciensJayaswal, R.K.; Veluthambi, K.; Gelvin, S.B.; Slightom, J.L.
-
Transgenic Populus: a step-by-step protocol for its Agrobacterium -mediated transformationTzfira, T.; Jensen, C.S.; Wangxia, W.; Zuker, A.; Altman, A.; Vainstein, A.
-
An Agrobacterium -mediated transient gene expression system for intact leavesKapila, J.; De Rycke, R.; Van Montagu, M.; Angenon, G.
-
Optimization of Agrobacterium -mediated transient assays of gene expression in lettuce, tomato and ArabidopsisWroblewski, T.; Tomczak, A.; Michelmore, R.
-
Plant Genetic Transformation and Gene ExpressionDraper, J.; Scott, R.; Armitage, P.; Walden, R.
-
Expression of genes transferred into monocot and dicot plant cells by electroporationFromm, M.; Taylor, L.P.; Walbot, V.
@Phil_Robichaud
@deepthiw
@JoseServera