Study of pharmacological effects of nilvadipine on RCS rat retinal degeneration by microarray analysis

Study of pharmacological effects of nilvadipine on RCS rat retinal degeneration by microarray... In our recent study, we found that the Ca 2+ antagonist, nilvadipine caused significant preservation of photoreceptor cells in The Royal College of Surgeons (RCS) rats (Invest. Ophthalmol. Vis. Sci. 43 (2002) 919). Here, to elucidate the mechanisms of nilvadipine-induced effects we analyzed altered gene expression of 1101 genes commonly expressed in rodent by DNA microarray analysis in the retinas of nilvadipine-treated and untreated RCS rats and SD rat. In the total number of genes, the expression of 30 genes was altered upon administration of nilvadipine to RCS rats, including several genes related to the apoptotic pathway and other mechanisms. Remarkably, neurotrophic factors, FGF-2 and Arc, known to suppress the apoptosis in the central nervous system, were up-regulated. These changes were also confirmed by real-time quantitative (Taqman) RT-PCR and Western blot analysis. Therefore, our present data suggested that administration of nilvadipine to RCS rats increases the expression of endogenous FGF-2 and Arc in retina, and potentially has a protective effect against retinal degeneration. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier Science (USA)
ISSN
0006-291x
D.O.I.
10.1016/S0006-291X(03)01092-1
Publisher site
See Article on Publisher Site

Abstract

In our recent study, we found that the Ca 2+ antagonist, nilvadipine caused significant preservation of photoreceptor cells in The Royal College of Surgeons (RCS) rats (Invest. Ophthalmol. Vis. Sci. 43 (2002) 919). Here, to elucidate the mechanisms of nilvadipine-induced effects we analyzed altered gene expression of 1101 genes commonly expressed in rodent by DNA microarray analysis in the retinas of nilvadipine-treated and untreated RCS rats and SD rat. In the total number of genes, the expression of 30 genes was altered upon administration of nilvadipine to RCS rats, including several genes related to the apoptotic pathway and other mechanisms. Remarkably, neurotrophic factors, FGF-2 and Arc, known to suppress the apoptosis in the central nervous system, were up-regulated. These changes were also confirmed by real-time quantitative (Taqman) RT-PCR and Western blot analysis. Therefore, our present data suggested that administration of nilvadipine to RCS rats increases the expression of endogenous FGF-2 and Arc in retina, and potentially has a protective effect against retinal degeneration.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Jul 11, 2003

References

  • Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat
    D’Cruz, P.M; Yasumura, D; Weir, J; Matthes, M.T; Abderrahim, H; LaVail, M.M; Vollrath, D
  • Molecular determinants of inactivation in voltage-gated Ca 2+ channels
    Hering, S; Berjukow, S; Sokolov, S; Marksteiner, R; Weiss, R.G; Klaus, R; Timin, E.N
  • Subunit and site-specific pharmacology of the NMDA receptor channel
    Yamakura, T; Shimoji, K
  • Molecular cloning and tissue distribution of rat peptide transporter PEPT2
    Saito, H; Terada, T; Okuda, M; Sasaki, K; Inui, S

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