Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica

Structural characterization and anticomplement activity of an acidic polysaccharide containing... A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom. Its structure was characterized by monosaccharide composition analysis, methylation, and NMR spectroscopy. It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→. The possible repetitive units were speculated and further analyzed by oligosaccharide analysis. YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL). Preliminary mechanism study indicated that it interacted with C1q, C2, C3, C4 and C5. These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Biological Macromolecules Elsevier

Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica

International Journal of Biological Macromolecules, Volume 132 – Jul 1, 2019

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Publisher
Elsevier
Copyright
Copyright © 2019 Elsevier Ltd
ISSN
0141-8130
D.O.I.
10.1016/j.ijbiomac.2019.04.029
Publisher site
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Abstract

A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom. Its structure was characterized by monosaccharide composition analysis, methylation, and NMR spectroscopy. It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→. The possible repetitive units were speculated and further analyzed by oligosaccharide analysis. YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL). Preliminary mechanism study indicated that it interacted with C1q, C2, C3, C4 and C5. These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.

Journal

International Journal of Biological MacromoleculesElsevier

Published: Jul 1, 2019

References

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