Access the full text.
Sign up today, get unlimited access with DeepDyve Pro!
, including Dicer and high mobility group A2 (Hmga2) [18]. Knockdown of lncRNA-H19 ameliorates LPS- induced apoptosis and inflammatory factor secretions by modulating miR-130a in osteoarthritis [19 ...
. Signal transduction pathways in Cyr61- induced MMP-3 production were examined by real-time PCR, western blotting, confocal microscopy, luciferase reporter assay. Mice with collagen - induced arthritis (CIA ...
responsible cells for this amelioration , our study showed that inducible PI3Kδ of FLSs is also involved in the activation of the PI3K-Akt pathway and synovial lining hyperplasia (Figure 4B and C ). In this study ...
group when compared with the PDGF-treated group (Figure 3A). The results indicated that JMJD3 mediated VSMCs migration induced by PDGF. Figure 3 View largeDownload slide Knockdown of JMJD3 or GSK J4 ...
knockdown of NLRP3. (E) Chondrocytes were pre-treated with PNU-282987 or NLRP3 siRNA for 2 h and then incubated with IL-1β. Relative protein levels of MMP-1, Collagen II, and MMP-13 in lysates of chondrocytes ...
, maintaining a final siRNA concentration of 0.1 μM. The cells were subsequently incubated for 5 hours at 37° C , allowing for siRNA uptake and gene knockdown . After this incubation period, we replenished the media ...
of MeCP2 in collagen expression. SiRNA - mediated knockdown of MeCP2 reduced collagen I expression but did not affect the levels of the myofibroblast marker α-smooth muscle actin (Fig. 5A). Fig. 5 View ...
. To ensure a good JAK knockdown efficiency of the analysed samples, we confirmed the siRNA and Jakinibs efficacy at both protein and mRNA levels [using target genes] [Supplementary Figure 7A– C and D ...
that DON- induced GRP78 degradation was cathepsin and calpain dependent but was proteosome-independent. RNAi- mediated knockdown of GRP78 resulted in increased IL-6 gene expression indicating a potential ...
of CD4+IL-17A+ T cells in the synovial fluid of animals with collagen - induced autoimmune arthritis [16]. Therefore we hypothesized that knockdown of Ctr9 could promote Th17 differentiation and worsen ...
Access the full text.
Sign up today, get unlimited access with DeepDyve Pro!