Single-step colony assay for screening antibody libraries

Single-step colony assay for screening antibody libraries •An scFv selection method was developed based on colony-filter screening.•The method does not involve transfer of filters with colonies before scFv expression.•Controlled exposure to an expression-inducing agent causes enhanced scFv expression.•Monoclonal scFvs were established using this single-step colony assay.•Reactivity of scFv clones established using our method was evaluated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biotechnology Elsevier

Single-step colony assay for screening antibody libraries

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0168-1656
eISSN
1873-4863
D.O.I.
10.1016/j.jbiotec.2017.06.010
Publisher site
See Article on Publisher Site

Abstract

•An scFv selection method was developed based on colony-filter screening.•The method does not involve transfer of filters with colonies before scFv expression.•Controlled exposure to an expression-inducing agent causes enhanced scFv expression.•Monoclonal scFvs were established using this single-step colony assay.•Reactivity of scFv clones established using our method was evaluated.

Journal

Journal of BiotechnologyElsevier

Published: Aug 10, 2017

References

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