Single pixel quantitative phase imaging with spatial frequency projections

Single pixel quantitative phase imaging with spatial frequency projections •Single pixel imaging is a powerful method that enables robust imaging in scattering media and integration of information not accessible to conventional camera imaging.•We demonstrate a method of common-path single pixel quantitative phase microscopy that opens the ability for phase imaging in new spectral regions, such as short wave infrared and mid infrared.•A general theory for single pixel intensity and quantitative phase microscopy is presented.•Simultaneous imaging of quantitative phase, absorption, and fluorescence through spatial frequency projections recorded on two separate detectors.•Single pixel quantitative phase images are presented in both forward and back scattered geometries, and quantitative images of absorption, phase, and fluorescence are shown in cultured cells.•Single pixel quantitative phase imaging is described with scattering theory to provide an intuitive understanding of the object spatial frequency information collected in both forward and back scattered geometries. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Methods Elsevier

Single pixel quantitative phase imaging with spatial frequency projections

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Publisher
Elsevier
Copyright
Copyright © 2017 The Authors
ISSN
1046-2023
eISSN
1095-9130
D.O.I.
10.1016/j.ymeth.2017.10.007
Publisher site
See Article on Publisher Site

Abstract

•Single pixel imaging is a powerful method that enables robust imaging in scattering media and integration of information not accessible to conventional camera imaging.•We demonstrate a method of common-path single pixel quantitative phase microscopy that opens the ability for phase imaging in new spectral regions, such as short wave infrared and mid infrared.•A general theory for single pixel intensity and quantitative phase microscopy is presented.•Simultaneous imaging of quantitative phase, absorption, and fluorescence through spatial frequency projections recorded on two separate detectors.•Single pixel quantitative phase images are presented in both forward and back scattered geometries, and quantitative images of absorption, phase, and fluorescence are shown in cultured cells.•Single pixel quantitative phase imaging is described with scattering theory to provide an intuitive understanding of the object spatial frequency information collected in both forward and back scattered geometries.

Journal

MethodsElsevier

Published: Mar 1, 2018

References

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