Simultaneous quantification of three alkylated‑purine adducts in human urine using sulfonic acid poly(glycidyl methacrylate‑divinylbenzene)-based microspheres as sorbent combined with LC-MS/MS

Simultaneous quantification of three alkylated‑purine adducts in human urine using sulfonic... Three alkylated DNA adducts, N3‑methyladenine, N3‑ethyladenine and N7‑ethylguanine, have been proved to be potential biomarkers for DNA injury caused by exposure to cigarette smoke. In this study, a highly specific and sensitive method using a new mixed-mode sulfonate-functionalized poly(glycidyl methacrylate-divinylbenzene) as a solid-phase extraction sorbent was developed for the analysis of these three alkylated-purine adducts in human urine. Under optimized conditions, the prepared sorbent interacts strongly with these urinary adducts, demonstrating high clean-up efficiency and extraction recovery. The method detection limits (S/N ≥ 3) of N3-MeA, N3-EtA and N7-EtG were 1.75, 0.20, and 0.15 pg mL−1, respectively, while the method quantitation limits were found to be 5.78, 0.66, and 0.49 pg mL−1 for N3-MeA, N3-EtA and N7-EtG, respectively. The intra-day and inter-day precisions were investigated, of which were in the range of 1.6–3.8% and 3.2–5.6%, respectively. The recovery values of the alkylated DNA adducts in spiked urine sample were ranged 89.7–104.5%. Their concentrations were statistically significantly higher in smokers than in nonsmokers. These results show that the proposed method is suitable for the analysis of alkylated DNA adducts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Chromatography B Elsevier

Simultaneous quantification of three alkylated‑purine adducts in human urine using sulfonic acid poly(glycidyl methacrylate‑divinylbenzene)-based microspheres as sorbent combined with LC-MS/MS

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
1570-0232
eISSN
1873-376X
D.O.I.
10.1016/j.jchromb.2018.02.028
Publisher site
See Article on Publisher Site

Abstract

Three alkylated DNA adducts, N3‑methyladenine, N3‑ethyladenine and N7‑ethylguanine, have been proved to be potential biomarkers for DNA injury caused by exposure to cigarette smoke. In this study, a highly specific and sensitive method using a new mixed-mode sulfonate-functionalized poly(glycidyl methacrylate-divinylbenzene) as a solid-phase extraction sorbent was developed for the analysis of these three alkylated-purine adducts in human urine. Under optimized conditions, the prepared sorbent interacts strongly with these urinary adducts, demonstrating high clean-up efficiency and extraction recovery. The method detection limits (S/N ≥ 3) of N3-MeA, N3-EtA and N7-EtG were 1.75, 0.20, and 0.15 pg mL−1, respectively, while the method quantitation limits were found to be 5.78, 0.66, and 0.49 pg mL−1 for N3-MeA, N3-EtA and N7-EtG, respectively. The intra-day and inter-day precisions were investigated, of which were in the range of 1.6–3.8% and 3.2–5.6%, respectively. The recovery values of the alkylated DNA adducts in spiked urine sample were ranged 89.7–104.5%. Their concentrations were statistically significantly higher in smokers than in nonsmokers. These results show that the proposed method is suitable for the analysis of alkylated DNA adducts.

Journal

Journal of Chromatography BElsevier

Published: Apr 1, 2018

References

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