•Representative indicators of fat soluble vitamins in the guidelines for medical diagnosis and treatment drafted by the Ministry of health could be simultaneously detection, suitable for the detection of fat soluble vitamins deficiencies.•This method with small sample volume lowers the probability of invalid detection because of insufficient sample size, which makes it an important application potential in pediatrics and other clinical examination.•Batched optimized pretreatment for sample could transferred into automatic operation easily.•μElution plate was used to lower the sample volume and simplify sample processing.
Journal of Chromatography B – Elsevier
Published: Mar 15, 2018
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