Metabolic Engineering 28 (2015) 8–18 Contents lists available at ScienceDirect Metabolic Engineering journal homepage: www.elsevier.com/locate/ymben Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae a a,b a a a,n Wenping Xie , Lidan Ye , Xiaomei Lv , Haoming Xu , Hongwei Yu Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, PR China Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, PR China a r ti cle in fo abstract Article history: Balanced utilization of metabolic intermediates and controllable expression of genes in biosynthetic Received 7 May 2014 pathways are key issues for the effective production of value-added chemicals in microbes. An inducer/ Received in revised form repressor-free sequential control strategy regulated by glucose concentration in the growth environment 24 October 2014 was proposed to address these issues, and its efﬁciency was validated using heterologous beta- Accepted 20 November 2014 carotenoid biosynthesis in Saccharomyces cerevisiae as an example. Through sequential control of the Available online 2 December 2014 downstream, upstream, and competitive pathways of farnesyl diphosphate (FPP), the crucial metabolic Keywords: node in the biosynthesis of terpenoids, in a predetermined order, a carotenoid
Metabolic Engineering – Elsevier
Published: Mar 1, 2015
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