Sequence analysis of a duck picornavirus isolate indicates that it together with porcine enterovirus type 8 and simian picornavirus type 2 should be assigned to a new picornavirus genus

Sequence analysis of a duck picornavirus isolate indicates that it together with porcine... In a 1990 outbreak, a virus isolated in Taiwan from the intestines of ducks showing signs of hepatitis was tentatively classified as a picornavirus on the basis of physical, chemical, and morphological characteristics. The virus was cloned and then found not to be type 1 duck hepatitis virus (DHV-1) or a new serotype of duck hepatitis virus (N-DHV) by serum neutralization. Complete genome sequencing indicated that the virus genome had 8351 nucleotides and the typical picornavirus genome organization (i.e., 5′ untranslated region (UTR)-L-P1 (VP 4-2-3-1)-P2 (2A-B-C)-P3 (3A-B-C-D)-3′ UTR-poly A). One open reading frame encoded 2521 amino acids, which makes this virus one of the largest picornaviruses, second only to equine rhinitis B virus of the genus Erbovirus . Its L protein was the largest within the family Picornaviridae (451 amino acids) and suspected to be a trypsin-like protease. The 235-nucleotide 3′ UTR region was of intermediate size, quite long compared to other picornaviruses but shorter than other picornaviruses of duck-origin (DHV-1 and N-DHV) and had four regions of secondary structure. The 2A protein was composed of only 12 amino acids, which is the shortest of any member of the family Picornaviridae . Phylogenetic analysis of the polyprotein and 3D sequences indicated that this virus (named duck picornavirus (DPV)) together with porcine enterovirus type 8 virus and several simian picornaviruses form a distinct branch of the family Picornaviridae and should be assigned to a new picornavirus genus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

Sequence analysis of a duck picornavirus isolate indicates that it together with porcine enterovirus type 8 and simian picornavirus type 2 should be assigned to a new picornavirus genus

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Publisher
Elsevier
Copyright
Copyright © 2007 Elsevier B.V.
ISSN
0168-1702
D.O.I.
10.1016/j.virusres.2007.06.023
Publisher site
See Article on Publisher Site

Abstract

In a 1990 outbreak, a virus isolated in Taiwan from the intestines of ducks showing signs of hepatitis was tentatively classified as a picornavirus on the basis of physical, chemical, and morphological characteristics. The virus was cloned and then found not to be type 1 duck hepatitis virus (DHV-1) or a new serotype of duck hepatitis virus (N-DHV) by serum neutralization. Complete genome sequencing indicated that the virus genome had 8351 nucleotides and the typical picornavirus genome organization (i.e., 5′ untranslated region (UTR)-L-P1 (VP 4-2-3-1)-P2 (2A-B-C)-P3 (3A-B-C-D)-3′ UTR-poly A). One open reading frame encoded 2521 amino acids, which makes this virus one of the largest picornaviruses, second only to equine rhinitis B virus of the genus Erbovirus . Its L protein was the largest within the family Picornaviridae (451 amino acids) and suspected to be a trypsin-like protease. The 235-nucleotide 3′ UTR region was of intermediate size, quite long compared to other picornaviruses but shorter than other picornaviruses of duck-origin (DHV-1 and N-DHV) and had four regions of secondary structure. The 2A protein was composed of only 12 amino acids, which is the shortest of any member of the family Picornaviridae . Phylogenetic analysis of the polyprotein and 3D sequences indicated that this virus (named duck picornavirus (DPV)) together with porcine enterovirus type 8 virus and several simian picornaviruses form a distinct branch of the family Picornaviridae and should be assigned to a new picornavirus genus.

Journal

Virus ResearchElsevier

Published: Nov 1, 2007

References

  • The novel mechanism of initiation of picornavirus RNA translation
    Jackson, R.J.; Howell, M.T.; Kaminski, A.
  • MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment
    Kumar, S.; Tamura, K.; Nei, M.
  • Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure
    Mathews, D.H.; Sabina, J.; Zuker, M.; Turner, D.H.
  • Molecular analysis of type 1 duck hepatitis virus indicated that it should be assigned to a new genus
    Tseng, C.H.; Knowles, N.J.; Tsai, H.J.
  • Molecular characterization of a new serotype of duck hepatitis virus
    Tseng, C.H.; Tsai, H.J.

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