Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl.

Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Efficient protocols were established for in vitro seed germination, neo -formation of secondary (2°) protocorms from primary (1°) protocorms and multiple shoot buds and protocorm-like body (PLB) induction from pseudo-stem segments of in vitro -raised seedlings of Cymbidium giganteum . Four nutrient media, namely Murashige and Skoog (MS), Phytamax (PM), Mitra et al. (M), and Knudson ‘C’ (KC) were evaluated for seed germination and early protocorm development. In addition, the effects of peptone, activated charcoal (AC) and two plant growth regulators (6-benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D)) were also studied. Both M and PM supplemented with 2.0 g l −1 peptone or 1.0 mg l −1 BAP resulted in ∼100% seed germination. Media supplemented with 2.0 g l −1 AC could effectively induce large protocorms (1.6 ± 0.1 mm in diameter). Neo -formation of 2° protocorms from 1° protocorms was achieved in liquid and agar-solidified PM medium fortified with different concentrations and combinations of auxins (α-naphthalene acetic acid (NAA) and 2,4-D) and cytokinins (BAP and kinetin (KN)). The highest number of 2° protocorms was obtained in liquid medium (10.7 ± 0.9/1° protocorm) supplemented with 2.0 mg l −1 BAP + 1.0 mg l −1 NAA. Although protocorms proliferated profusely in liquid medium, these did not develop further unless transferred to agar-solidified medium within 6–8 weeks. Multiple shoot buds and PLBs were induced from pseudo-stem segments on agar-solidified PM medium fortified with different concentrations and combinations of BAP and NAA and the maximum number of PLBs (6.00 ± 0.20) was recorded when BAP and NAA were applied at 2.0 mg l −1 each. A solid root system was induced from PLBs and shoot buds when these were transferred to half-strength PM or M media fortified with 0.5 mg l −1 indole-3-acetic acid. Well-rooted plants were transferred to the greenhouse with 95% survival. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Scientia Horticulturae Elsevier

Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl.

Loading next page...
 
/lp/elsevier/seed-germination-and-tissue-culture-of-cymbidium-giganteum-wall-ex-Q3h6Zq0AZV
Publisher
Elsevier
Copyright
Copyright © 2009 Elsevier B.V.
ISSN
0304-4238
eISSN
1879-1018
D.O.I.
10.1016/j.scienta.2009.10.009
Publisher site
See Article on Publisher Site

Abstract

Efficient protocols were established for in vitro seed germination, neo -formation of secondary (2°) protocorms from primary (1°) protocorms and multiple shoot buds and protocorm-like body (PLB) induction from pseudo-stem segments of in vitro -raised seedlings of Cymbidium giganteum . Four nutrient media, namely Murashige and Skoog (MS), Phytamax (PM), Mitra et al. (M), and Knudson ‘C’ (KC) were evaluated for seed germination and early protocorm development. In addition, the effects of peptone, activated charcoal (AC) and two plant growth regulators (6-benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D)) were also studied. Both M and PM supplemented with 2.0 g l −1 peptone or 1.0 mg l −1 BAP resulted in ∼100% seed germination. Media supplemented with 2.0 g l −1 AC could effectively induce large protocorms (1.6 ± 0.1 mm in diameter). Neo -formation of 2° protocorms from 1° protocorms was achieved in liquid and agar-solidified PM medium fortified with different concentrations and combinations of auxins (α-naphthalene acetic acid (NAA) and 2,4-D) and cytokinins (BAP and kinetin (KN)). The highest number of 2° protocorms was obtained in liquid medium (10.7 ± 0.9/1° protocorm) supplemented with 2.0 mg l −1 BAP + 1.0 mg l −1 NAA. Although protocorms proliferated profusely in liquid medium, these did not develop further unless transferred to agar-solidified medium within 6–8 weeks. Multiple shoot buds and PLBs were induced from pseudo-stem segments on agar-solidified PM medium fortified with different concentrations and combinations of BAP and NAA and the maximum number of PLBs (6.00 ± 0.20) was recorded when BAP and NAA were applied at 2.0 mg l −1 each. A solid root system was induced from PLBs and shoot buds when these were transferred to half-strength PM or M media fortified with 0.5 mg l −1 indole-3-acetic acid. Well-rooted plants were transferred to the greenhouse with 95% survival.

Journal

Scientia HorticulturaeElsevier

Published: Feb 2, 2010

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off