Polyphenols are chemicals that primarily inhibit the growth of various autotrophic organisms. The presence of these metabolites greatly boosts the ecological dominance of eg. Cladophora, which creates large surface mats. The main goal of our work was to quantify the phenol and polyphenols (allelopathic substances) secreted by the macroalgae as a result of exposure to biotic stress caused by competition. The research was carried out on the Cladophora glomerata biomass collected from two freshwater ecosystems located in Wielkopolska Region (Poland, Fig. 1): Oporzynskie Lake (N52° 55′, E 17° 9′) and Nielba River (N52˚48′, E 17° 12′). Seasonal variability (May-October 2015) in the properties of C. glomerata mats in the river and lake ecosystem was also analyzed in relation to the physicochemical parameters of water. In addition, the content of pigments in the analyzed biomass was determined during the appearance of algae mats in water reservoirs. Biomass extraction was performed to determine the phenolic and carotenoid contents (chlorophyll and carotenoids) by using two extraction methods: microwave-assisted extraction (MAE) and supercritical fluid extraction (SFE). After isolation of the phenols from the thalli (mats) and the habitat, they were analyzed using the Folin-Ciocalteu method with some modifications, while the pigment content (chlorophyll and carotenoids) was evaluated by the spectrophotometric method Liechtenthaler (1987) with some variations. Analysis of the content of these components in algae extracts indicates that the tendency of changes in their contents was similar or the same. Growth and decrease of phenolic content (Cladophora T MAE and Cladophora T SFE) and pigments (MAE chlorophyll, SFE and MAE carotenoids) at the same time were independent of the insulation method used. The mats formed by C. glomerata on the surface of Lake Oporzyńskie were more stable and larger surface area than those on the Nielba River, which could explain differences in polyphenol concentrations in these two aquatic ecosystems. The results suggest a reduction in the secretion of phenolic compounds with an aging population of algae.
Water Research – Elsevier
Published: May 15, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera