Response of the bacterial community in an on-farm biopurification system, to which diverse pesticides are introduced over an agricultural season

Response of the bacterial community in an on-farm biopurification system, to which diverse... A biopurification system (BPS) is used on-farm to clean pesticide-contaminated wastewater. Due to high pesticide loads, a BPS represents a hot spot for the proliferation and selection as well as the genetic adaptation of discrete pesticide degrading microorganisms. However, while considerable knowledge exists on the biodegradation of specific pesticides in BPSs, the bacterial community composition of these systems has hardly been explored. In this work, the Shannon diversity, the richness and the composition of the bacterial community within an operational BPS receiving wastewater contaminated with various pesticides was, for the first time, elucidated over the course of an agricultural season, using DGGE profiling and pyrosequencing of 16S rRNA gene fragments amplified from total community DNA. During the agricultural season, an increase in the concentration of pesticides in the BPS was observed along with the detection of significant community changes including a decrease in microbial diversity. Additionally, a significant increase in the relative abundance of Proteobacteria, mainly the Gammaproteobacteria, was found, and OTUs (operational taxonomic units) affiliated to Pseudomonas responded positively during the course of the season. Furthermore, a banding-pattern analysis of 16S rRNA gene-based DGGE fingerprinting, targeting the Alpha- and Betaproteobacteria as well as the Actinobacteria, indicated that the Betaproteobacteria might play an important role. Interestingly, a decrease of Firmicutes and Bacteroidetes was observed, indicating their selective disadvantage in a BPS, to which pesticides have been introduced. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Pollution Elsevier

Response of the bacterial community in an on-farm biopurification system, to which diverse pesticides are introduced over an agricultural season

Response of the bacterial community in an on-farm biopurification system, to which diverse pesticides are introduced over an agricultural season

Environmental Pollution 229 (2017) 854e862 Contents lists available at ScienceDirect Environmental Pollution journal homepage: www.elsevier.com/locate/envpol Response of the bacterial community in an on-farm biopurification system, to which diverse pesticides are introduced over an agricultural season a, 1 b, c, 1 d b Peter N. Holmsgaard , Simone Dealtry , Vincent Dunon , Holger Heuer , a, e d b a, * a Lars H. Hansen , Dirk Springael , Kornelia Smalla , Leise Riber , Søren J. Sørensen Section for Microbiology, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark Julius Kühn-Institut - Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, 38104 Braunschweig, Germany ^ ~ Civil Engineering Department, Catholic University of Rio de Janeiro, Rua Marques de Sao Vicente 225/301-L, Gavea, 22453-900 Rio de Janeiro, RJ, Brazil Division of Soil and Water Management, KULeuven, 3001 Leuven, Belgium Department of Environmental Science, Aarhus University, Frederiksborgvej 399, DK-4000 Roskilde, Denmark article i nf o abstract Article history: A biopurification system (BPS) is used on-farm to clean pesticide-contaminated wastewater. Due to high Received 7 March 2017 pesticide loads, a BPS represents a hot spot for the proliferation and selection as well as the genetic Received in revised form adaptation of discrete pesticide degrading microorganisms. However, while considerable knowledge 6 July 2017 exists on the biodegradation of specific pesticides in BPSs, the bacterial community composition of these Accepted 8 July 2017 systems has hardly been explored. In this work, the Shannon diversity, the richness and the composition Available online 20 July 2017 of the bacterial community within an operational BPS receiving wastewater contaminated with various pesticides was, for the first time, elucidated over the course of an agricultural season, using DGGE...
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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0269-7491
D.O.I.
10.1016/j.envpol.2017.07.026
Publisher site
See Article on Publisher Site

Abstract

A biopurification system (BPS) is used on-farm to clean pesticide-contaminated wastewater. Due to high pesticide loads, a BPS represents a hot spot for the proliferation and selection as well as the genetic adaptation of discrete pesticide degrading microorganisms. However, while considerable knowledge exists on the biodegradation of specific pesticides in BPSs, the bacterial community composition of these systems has hardly been explored. In this work, the Shannon diversity, the richness and the composition of the bacterial community within an operational BPS receiving wastewater contaminated with various pesticides was, for the first time, elucidated over the course of an agricultural season, using DGGE profiling and pyrosequencing of 16S rRNA gene fragments amplified from total community DNA. During the agricultural season, an increase in the concentration of pesticides in the BPS was observed along with the detection of significant community changes including a decrease in microbial diversity. Additionally, a significant increase in the relative abundance of Proteobacteria, mainly the Gammaproteobacteria, was found, and OTUs (operational taxonomic units) affiliated to Pseudomonas responded positively during the course of the season. Furthermore, a banding-pattern analysis of 16S rRNA gene-based DGGE fingerprinting, targeting the Alpha- and Betaproteobacteria as well as the Actinobacteria, indicated that the Betaproteobacteria might play an important role. Interestingly, a decrease of Firmicutes and Bacteroidetes was observed, indicating their selective disadvantage in a BPS, to which pesticides have been introduced.

Journal

Environmental PollutionElsevier

Published: Oct 1, 2017

References

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