Repellent and insecticidal activities of essential oils from
Artemisia princeps and Cinnamomum camphora and their eﬀect on
seed germination of wheat and broad bean
, A.K. Mishra
, R.X. Tan, C. Tang, H. Yang, Y.F. Shen
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,
Nanjing 210093, PR China
Received 31 May 2005; received in revised form 1 September 2005; accepted 3 September 2005
Available online 17 October 2005
Repellent and insecticidal activities of essential oils extracted from leaves of Artemisia princeps Pamp and seeds of Cinnamomum cam-
phora (L.) Presl. against storage pests Sitophillus oryzae L. and Bruchus rugimanus Bohem were investigated. Results showed that the two
individual oils displayed good, but their mixture (1:1) exhibited much better repellent activities at concentrations from 250 to 1000 lgg
and insecticidal actions at concentrations P1000 lgg
against the test beetles S. oryzae and B. rugimanus. Oils from A. princeps and C.
camphora applied individually were signiﬁcantly toxic to seed germination of wheat at 500 lgml
. However, no toxic eﬀects were found
when the two oils were mixed (1:1 w/w) at the same concentration. These observations indicated that the mixture of the two plant-derived
oils had a synergic eﬀect and could be used in the control of storage pests.
Ó 2005 Elsevier Ltd. All rights reserved.
Keywords: Essential oils; Artimisia princeps; Cinnamomum camphora; Insecticidal; Phytotoxic
Essential oils, naturally occurring substances, generally
have a broad spectrum of bioactivity because of the pres-
ence of several active ingredients that work through several
modes of action. The toxicity of individual oils or com-
pounds often exerts diﬀerential eﬀects depending on both
the mode of action and the target pest (Isman, 2000). Re-
cently, as an alternative pest control technology, essential
oils are attracted particular attention because of their speci-
ﬁcity to pests, biodegradable nature and potential for com-
Artemisia princeps Pamp (Asteraceae) and Cinnamomum
camphora (L.) Presl. (Lauraceae) are Chinese medicinal
plants widespread in China. The essential oils, compounds
and their antimicrobial, insecticidal as well as repellent
activities of both Artemisia and Cinnamomum have been re-
ported (Kim et al., 2003; Liu et al., 2001; Teixeira da Silva,
2004; Tripathi et al., 2000; Wang et al., 2005), but their syn-
ergetic eﬀect of the oils from the two plants has not been
acknowledged. The present study was a continuation of
our previous research on the chemical composition and
antimicrobial activities of A. princeps and C. camphora
(Liu et al., 2001) and mainly aimed at a study of their phy-
totoxic, insecticidal activities and their synergistic eﬀects in
order to explore a novel natural chemotherapeutic agent
for the control of stored grain pests.
Mature leaves of A. princeps and seeds of C. camphora
were collected from Nanjing, China and their essential oils
0960-8524/$ - see front matter Ó 2005 Elsevier Ltd. All rights reserved.
Corresponding author. Tel.: +86 25 83685469.
E-mail addresses: firstname.lastname@example.org, email@example.com (C.H. Liu).
Visiting scholar from the Department of Botany, K.N. Govt. P.G.
College, Gyanpur 221 304 UP, India.
Bioresource Technology 97 (2006) 1969–1973