Repeated fine-needle aspiration of solid tumours in mice allows the identification of multiple infiltrating immune cell types

Repeated fine-needle aspiration of solid tumours in mice allows the identification of multiple... This paper describes a novel method for following the changes in mouse tumour-infiltrating immune cell populations by repeated sampling of tumours by fine needle aspiration (FNA), followed by flow cytometry. Using this technique we were able to collect samples from P815 mouse mastocytomas, and identify and enumerate six tumour-infiltrating immune cell types at multiple time points for each mouse. We demonstrate good agreement between cell percentages obtained from FNA samples and matched whole tumour digests (WTDs). We also demonstrate that neither survival nor the incidence of liver metastasis is adversely affected by the procedure. Our method has a clear advantage over the common practice of sacrificing mice and collecting tissue at pre-determined time points, as the technique allows 1) repeated sampling of each mouse over time, thus many fewer mice are required, and 2) the correlation of survival data with tumour-infiltrating immune cell types at different time points. This potentially allows immune cell types associated with increased or decreased survival to be identified. Therefore, our technique should greatly facilitate the characterisation of anti-tumour immunity induced in response to cancer therapy in small animal models. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Immunological Methods Elsevier

Repeated fine-needle aspiration of solid tumours in mice allows the identification of multiple infiltrating immune cell types

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Publisher
Elsevier
Copyright
Copyright © 2015 Elsevier Ltd
ISSN
0022-1759
D.O.I.
10.1016/j.jim.2015.06.015
Publisher site
See Article on Publisher Site

Abstract

This paper describes a novel method for following the changes in mouse tumour-infiltrating immune cell populations by repeated sampling of tumours by fine needle aspiration (FNA), followed by flow cytometry. Using this technique we were able to collect samples from P815 mouse mastocytomas, and identify and enumerate six tumour-infiltrating immune cell types at multiple time points for each mouse. We demonstrate good agreement between cell percentages obtained from FNA samples and matched whole tumour digests (WTDs). We also demonstrate that neither survival nor the incidence of liver metastasis is adversely affected by the procedure. Our method has a clear advantage over the common practice of sacrificing mice and collecting tissue at pre-determined time points, as the technique allows 1) repeated sampling of each mouse over time, thus many fewer mice are required, and 2) the correlation of survival data with tumour-infiltrating immune cell types at different time points. This potentially allows immune cell types associated with increased or decreased survival to be identified. Therefore, our technique should greatly facilitate the characterisation of anti-tumour immunity induced in response to cancer therapy in small animal models.

Journal

Journal of Immunological MethodsElsevier

Published: Oct 1, 2015

References

  • A late look at the safety of aspiration biopsy
    Berg, J.W.; Robbins, G.F.
  • Surface expression of CD63 antigen (AD1 Antigen) in P815 mastocytoma cells by transfected IgE receptors
    Furuno, T.; Teshima, R.; Kitani, S.; Sawada, J.-I.; Nakanishi, M.
  • Is aspiration biopsy of breast cancer dangerous to the patient?
    Robbins, G.F.; Brothers, J.H.; Eberhart, W.F.; Quan, S.
  • Minimally invasive assessment of tumor angiogenesis by fine needle aspiration and flow cytometry
    Sottnik, J.; Guth, A.; Mitchell, L.; Dow, S.
  • Natural innate and adaptive immunity to cancer
    Vesely, M.D.; Kershaw, M.H.; Schreiber, R.D.; Smyth, M.J.

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