Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit

Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and... Fruit reflectance spectra of five apple ( Malus domestica Borkh.) cultivars (Zhigulevskoe, Antonovka, Granny Smith, Golden Delicious and Renet Simirenko) with a wide range of peel pigment (chlorophylls a and b , carotenoids and anthocyanins) content have been studied to develop non-destructive techniques for pigment assessment. In addition to chlorophylls, positions of in vivo absorption maxima were established for carotenoids (480, 455 and a shoulder at 425 nm) and for anthocyanins (near 550 nm). In anthocyanin-free fruit, a close relationship between reflectance at 550 nm (R 550 ) and 700 nm (R 700 ) has been found ( r 2 >0.95). In fruit with chlorophyll content more than 5 nmol/cm 2 , the reflectance near 678 nm was insensitive to a variation in chlorophylls, whereas, reflectance in the bands 550–650 nm and 690–705 nm remained sensitive to chlorophyll content in a wide range of its variation. The reflectance ratios, R 800 /R 700 and R 800 /R 640 , were directly proportional to total chlorophyll content ranging from 0.4 to 11 nmol/cm 2 ( r 2 >0.93). The reflectance in the band 520–530 nm was found to be dependent mostly on carotenoids absorption. The index R 800 (1/R 520 −1/R 700 ) was suggested for estimation of carotenoid content in the range 0.6–4.5 nmol/cm 2 . The index for assessment of a carotenoid/chlorophyll ratio was proposed in the form, (R 480 −R 678 )/R 800 . Reflectance in the green region of the spectrum proved to be sensitive to anthocyanin content. The index R 800 (1/R 550 −1/R 700 ) was developed to estimate anthocyanin content in peel ranging from 2.5 to 50 nmol/cm 2 ; the determination coefficient of the index with anthocyanin content was higher than 0.93. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Postharvest Biology and Technology Elsevier

Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science B.V.
ISSN
0925-5214
DOI
10.1016/S0925-5214(02)00066-2
Publisher site
See Article on Publisher Site

Abstract

Fruit reflectance spectra of five apple ( Malus domestica Borkh.) cultivars (Zhigulevskoe, Antonovka, Granny Smith, Golden Delicious and Renet Simirenko) with a wide range of peel pigment (chlorophylls a and b , carotenoids and anthocyanins) content have been studied to develop non-destructive techniques for pigment assessment. In addition to chlorophylls, positions of in vivo absorption maxima were established for carotenoids (480, 455 and a shoulder at 425 nm) and for anthocyanins (near 550 nm). In anthocyanin-free fruit, a close relationship between reflectance at 550 nm (R 550 ) and 700 nm (R 700 ) has been found ( r 2 >0.95). In fruit with chlorophyll content more than 5 nmol/cm 2 , the reflectance near 678 nm was insensitive to a variation in chlorophylls, whereas, reflectance in the bands 550–650 nm and 690–705 nm remained sensitive to chlorophyll content in a wide range of its variation. The reflectance ratios, R 800 /R 700 and R 800 /R 640 , were directly proportional to total chlorophyll content ranging from 0.4 to 11 nmol/cm 2 ( r 2 >0.93). The reflectance in the band 520–530 nm was found to be dependent mostly on carotenoids absorption. The index R 800 (1/R 520 −1/R 700 ) was suggested for estimation of carotenoid content in the range 0.6–4.5 nmol/cm 2 . The index for assessment of a carotenoid/chlorophyll ratio was proposed in the form, (R 480 −R 678 )/R 800 . Reflectance in the green region of the spectrum proved to be sensitive to anthocyanin content. The index R 800 (1/R 550 −1/R 700 ) was developed to estimate anthocyanin content in peel ranging from 2.5 to 50 nmol/cm 2 ; the determination coefficient of the index with anthocyanin content was higher than 0.93.

Journal

Postharvest Biology and TechnologyElsevier

Published: Feb 1, 2003

References

  • Quality measurement of fruit and vegetables
    Abbott, J.A.
  • The carotenoids as anti-oxidants
    Edge, R.; McGarvey, D.J.; Truscotte, T.G.
  • Light-induced pigment changes and evidence for anthocyanin photoprotection in apples
    Merzlyak, M.N.; Chivkunova, O.B.
  • Vacuolar contents of fruit subepidermal cells from Vitis species
    Moskowitz, A.; Hrazdina, G.

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