Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes

Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated... IntroductionTrachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), is the leading infectious cause of preventable blindness. Many commercial platforms are available that provide highly sensitive and specific detection of Ct DNA. However, the majority of these commercial platforms are inaccessible for population-level surveys in resource-limited settings typical to trachoma control programmes. We developed two low-cost quantitative PCR (qPCR) tests for Ct using readily available reagents on standard real-time thermocyclers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal Of Microbiological Methods Elsevier

Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0167-7012
eISSN
1872-8359
D.O.I.
10.1016/j.mimet.2017.04.010
Publisher site
See Article on Publisher Site

Abstract

IntroductionTrachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), is the leading infectious cause of preventable blindness. Many commercial platforms are available that provide highly sensitive and specific detection of Ct DNA. However, the majority of these commercial platforms are inaccessible for population-level surveys in resource-limited settings typical to trachoma control programmes. We developed two low-cost quantitative PCR (qPCR) tests for Ct using readily available reagents on standard real-time thermocyclers.

Journal

Journal Of Microbiological MethodsElsevier

Published: Aug 1, 2017

References

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