Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan

Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl... •A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses.•This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre.•No direct correlation was found with viral load and symptom severity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2017.05.012
Publisher site
See Article on Publisher Site

Abstract

•A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses.•This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre.•No direct correlation was found with viral load and symptom severity.

Journal

Journal of Virological MethodsElsevier

Published: Oct 1, 2017

References

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