Reaction mixture analysis by ESI-MS: Mercury(II) and dicysteinyl tripeptide complex formation

Reaction mixture analysis by ESI-MS: Mercury(II) and dicysteinyl tripeptide complex formation Article history: Current clinical treatment for mercury poisoning generally depends on the complexation of mercury ions Received 21 October 2017 with dithiol compounds. Although mercury is known to have a high binding affinity for the soft sulfur Received in revised form 14 January 2018 donor atoms, further understanding of its complex formation tendencies in the presence of auxiliary Accepted 14 January 2018 donor atoms is necessary to enhance its immobilization and detoxification. In order to evaluate the effect Available online 31 January 2018 of auxiliary binding groups on mercury complex formation, two dicysteinyl tripeptides, CXC, where C is cysteine and X is glycine (CGC) or glutamic acid (CEC) were reacted with mercury(II) chloride. These Keywords: peptides provide the structural differences to evaluate the role of an added gamma carboxylate group in Cysteinyl peptides complex formation. The reaction mixtures were studied by Electrospray Ionization Mass Spectrometry ESI mass spectrometry (ESI-MS). Low micromolar mercury(II) solutions consisting of mercury(II) to peptide molar ratio of 1:2, Mercury(II)–peptide complexes 1:1, and 1:0.5 were analyzed for changes in complex speciation after mixing. The results show that the Mercury isotopic patterns major complex formed is the 1:1 Hg(peptide) complex for both dithiol peptide ligands. Other complexes http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Mass Spectrometry Elsevier

Reaction mixture analysis by ESI-MS: Mercury(II) and dicysteinyl tripeptide complex formation

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
1387-3806
eISSN
1873-2798
D.O.I.
10.1016/j.ijms.2018.01.010
Publisher site
See Article on Publisher Site

Abstract

Article history: Current clinical treatment for mercury poisoning generally depends on the complexation of mercury ions Received 21 October 2017 with dithiol compounds. Although mercury is known to have a high binding affinity for the soft sulfur Received in revised form 14 January 2018 donor atoms, further understanding of its complex formation tendencies in the presence of auxiliary Accepted 14 January 2018 donor atoms is necessary to enhance its immobilization and detoxification. In order to evaluate the effect Available online 31 January 2018 of auxiliary binding groups on mercury complex formation, two dicysteinyl tripeptides, CXC, where C is cysteine and X is glycine (CGC) or glutamic acid (CEC) were reacted with mercury(II) chloride. These Keywords: peptides provide the structural differences to evaluate the role of an added gamma carboxylate group in Cysteinyl peptides complex formation. The reaction mixtures were studied by Electrospray Ionization Mass Spectrometry ESI mass spectrometry (ESI-MS). Low micromolar mercury(II) solutions consisting of mercury(II) to peptide molar ratio of 1:2, Mercury(II)–peptide complexes 1:1, and 1:0.5 were analyzed for changes in complex speciation after mixing. The results show that the Mercury isotopic patterns major complex formed is the 1:1 Hg(peptide) complex for both dithiol peptide ligands. Other complexes

Journal

International Journal of Mass SpectrometryElsevier

Published: Mar 1, 2018

References

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