Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch

Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch •Optimal parameters for generating stable inducible CHO pools expressing recombinant proteins were identified.•The CHO stable pool generation process is robust and can lead to pools expressing up to 800mg/L of r-proteins in a fed-batch process.•The pools are stable for over one month in culture and can be cryopreserved without losing productivity upon thawing.•Pools made with the cumate expression system outperform those generated with the CMV or the EF1a-HTLV hybrid promoters. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biotechnology Elsevier

Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0168-1656
eISSN
1873-4863
D.O.I.
10.1016/j.jbiotec.2017.06.009
Publisher site
See Article on Publisher Site

Abstract

•Optimal parameters for generating stable inducible CHO pools expressing recombinant proteins were identified.•The CHO stable pool generation process is robust and can lead to pools expressing up to 800mg/L of r-proteins in a fed-batch process.•The pools are stable for over one month in culture and can be cryopreserved without losing productivity upon thawing.•Pools made with the cumate expression system outperform those generated with the CMV or the EF1a-HTLV hybrid promoters.

Journal

Journal of BiotechnologyElsevier

Published: Aug 10, 2017

References

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