Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species

Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species The random amplified polymorphic DNA (RAPD) method was used to generate fingerprint patterns for 10 meat species: wild boar, pig, horse, buffalo, beef, venison, dog, cat, rabbit and kangaroo. A total of 29 10-nucleotide primers, with GC contents ranging from 50–80%, were evaluated for their specificity and efficiency. The fingerprint patterns that were generated were found in some cases to be species-specific, i.e. one species could be differentiated from another. The advantages and disadvantages of using RAPD-PCR for the identification of red meat species are also discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Meat Science Elsevier

Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species

Meat Science, Volume 48 (3) – Mar 1, 1998

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Publisher
Elsevier
Copyright
Copyright © 1998 Elsevier Ltd
ISSN
0309-1740
D.O.I.
10.1016/S0309-1740(97)00104-6
Publisher site
See Article on Publisher Site

Abstract

The random amplified polymorphic DNA (RAPD) method was used to generate fingerprint patterns for 10 meat species: wild boar, pig, horse, buffalo, beef, venison, dog, cat, rabbit and kangaroo. A total of 29 10-nucleotide primers, with GC contents ranging from 50–80%, were evaluated for their specificity and efficiency. The fingerprint patterns that were generated were found in some cases to be species-specific, i.e. one species could be differentiated from another. The advantages and disadvantages of using RAPD-PCR for the identification of red meat species are also discussed.

Journal

Meat ScienceElsevier

Published: Mar 1, 1998

References

  • Identification of the species of origin of fresh, cooked and canned meat on meat products using antisera to thermostable muscle antigens by Ouchterlony's double diffusion test
    Kang'ethe, E.K.; Gathuma, J.M.; Lindqvist, K.J.

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