Proteolysis of whey proteins by a Bacillus subtilis enzyme preparation

Proteolysis of whey proteins by a Bacillus subtilis enzyme preparation Proteins in acid whey were hydrolysed by an enzymatic preparation from Bacillus subtilis , as a possible treatment that may reduce their allergenicity. The degree of hydrolysis at different conditions of pH, temperature and enzyme-substrate ratio was determined. Operating conditions that did not involve production of bitter peptides were identified. The selected hydrolysis conditions resulted in a substantial disappearance of α-lactalbumin and β-lactoglobulin from the polyacrylamide gel electrophoresis patterns. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Dairy Journal Elsevier

Proteolysis of whey proteins by a Bacillus subtilis enzyme preparation

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0958-6946
eISSN
1879-0143
D.O.I.
10.1016/0958-6946(95)00024-0
Publisher site
See Article on Publisher Site

Abstract

Proteins in acid whey were hydrolysed by an enzymatic preparation from Bacillus subtilis , as a possible treatment that may reduce their allergenicity. The degree of hydrolysis at different conditions of pH, temperature and enzyme-substrate ratio was determined. Operating conditions that did not involve production of bitter peptides were identified. The selected hydrolysis conditions resulted in a substantial disappearance of α-lactalbumin and β-lactoglobulin from the polyacrylamide gel electrophoresis patterns.

Journal

International Dairy JournalElsevier

Published: Mar 1, 1996

References

  • Immunogenicity and allergenicity of whey protein hydrolysates
    Asselin, J.; Amiot, J.; Gauthier, S.J.; Mourand, W.; Herbert, J.
  • Effects of in vitro proteolysis on the allergenicity of major whey proteins
    Asselin, J.; Herbert, J.; Amiot, J.

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