Protective effect of epigallocatechin gallate on brain damage after transient middle cerebral artery occlusion in rats

Protective effect of epigallocatechin gallate on brain damage after transient middle cerebral... Epigallocatechin gallate (EGCG), a major constituent of green tea, is a potent free radical scavenger. The purpose of this study was to verify whether EGCG reduces focal ischemia/reperfusion-induced brain injury in a rat model. Male Sprague–Dawley rats were anesthetized with chloral hydrate (400 mg/kg, i.p.) and subjected to a middle cerebral artery 2 h occlusion and then a 24-h reperfusion. The EGCG (25 mg and 50 mg/kg, i.p.) or vehicle was administered immediately after reperfusion. Twenty-four hours after reperfusion, infarction size, levels of oxidative stress markers (malondialdehyde and oxidized/total glutathione ratio) in the brain and neurological deficits were evaluated. The dose of 50 mg/kg of EGCG significantly reduced the infarction volume (9.9±3.2%) as compared to those (45.6±5.3%, 34.5±7.8%) of the control group and the EGCG 25 mg/kg treated group ( p <0.01). The dose of 50 mg/kg of EGCG significantly reduced the neurological deficit total score (5.2±1.7) as compared to those (9.5±1.2, 8.5±2.5) of the control group and the EGCG 25 mg/kg treated group ( p <0.05). The dose of 50 mg/kg of EGCG significantly attenuated the level of malondialdehyde and the level of oxidized/total glutathione ratio (281±66 nmol/g and 0.48±0.03) as compared to the those (415±46 nmol/g and 0.64±0.05, 381±51 nmol/g and 0.61±0.06) of the control group and the EGCG 25 mg/kg treated group ( p <0.05). These results demonstrate the anti-oxidant effects of EGCG (50 mg/kg) in a rat model of transient focal ischemia, which is a likely explanation for EGCG's neuroprotective effects. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Protective effect of epigallocatechin gallate on brain damage after transient middle cerebral artery occlusion in rats

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Publisher
Elsevier
Copyright
Copyright © 2004 Elsevier B.V.
ISSN
0006-8993
DOI
10.1016/j.brainres.2004.05.079
Publisher site
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Abstract

Epigallocatechin gallate (EGCG), a major constituent of green tea, is a potent free radical scavenger. The purpose of this study was to verify whether EGCG reduces focal ischemia/reperfusion-induced brain injury in a rat model. Male Sprague–Dawley rats were anesthetized with chloral hydrate (400 mg/kg, i.p.) and subjected to a middle cerebral artery 2 h occlusion and then a 24-h reperfusion. The EGCG (25 mg and 50 mg/kg, i.p.) or vehicle was administered immediately after reperfusion. Twenty-four hours after reperfusion, infarction size, levels of oxidative stress markers (malondialdehyde and oxidized/total glutathione ratio) in the brain and neurological deficits were evaluated. The dose of 50 mg/kg of EGCG significantly reduced the infarction volume (9.9±3.2%) as compared to those (45.6±5.3%, 34.5±7.8%) of the control group and the EGCG 25 mg/kg treated group ( p <0.01). The dose of 50 mg/kg of EGCG significantly reduced the neurological deficit total score (5.2±1.7) as compared to those (9.5±1.2, 8.5±2.5) of the control group and the EGCG 25 mg/kg treated group ( p <0.05). The dose of 50 mg/kg of EGCG significantly attenuated the level of malondialdehyde and the level of oxidized/total glutathione ratio (281±66 nmol/g and 0.48±0.03) as compared to the those (415±46 nmol/g and 0.64±0.05, 381±51 nmol/g and 0.61±0.06) of the control group and the EGCG 25 mg/kg treated group ( p <0.05). These results demonstrate the anti-oxidant effects of EGCG (50 mg/kg) in a rat model of transient focal ischemia, which is a likely explanation for EGCG's neuroprotective effects.

Journal

Brain ResearchElsevier

Published: Sep 3, 2004

References

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