Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals

Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced... The ability of oxidative stress to induce apoptosis, and the effect of Ginkgo biloba extract (EGb761) on this induction were studied in primary cultured rat cerebellar neuronal cells. Cells were exposed to hydroxyl radicals by treating them with 20–50 μM hydrogen peroxide (H 2 O 2 ) and 100 μM ferrous sulfate. Hydroxyl radical treatment fragmented the DNA in a manner typical or apoptosis cells, producing a ladder pattern of 200 base pair increments on 1% agarose gel electrophoresis. Pretreatment of cells with 100 μg/ml EGb reduced hydroxyl radical induced cells apoptosis (determined by flow cytometry) and DNA fragmentation. The results indicate that hydroxyl radicals induce apoptosis in rat cerebellar neuronal cells, and this induction can be prevented by EGb. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuroscience Letters Elsevier

Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0304-3940
D.O.I.
10.1016/0304-3940(96)12897-4
Publisher site
See Article on Publisher Site

Abstract

The ability of oxidative stress to induce apoptosis, and the effect of Ginkgo biloba extract (EGb761) on this induction were studied in primary cultured rat cerebellar neuronal cells. Cells were exposed to hydroxyl radicals by treating them with 20–50 μM hydrogen peroxide (H 2 O 2 ) and 100 μM ferrous sulfate. Hydroxyl radical treatment fragmented the DNA in a manner typical or apoptosis cells, producing a ladder pattern of 200 base pair increments on 1% agarose gel electrophoresis. Pretreatment of cells with 100 μg/ml EGb reduced hydroxyl radical induced cells apoptosis (determined by flow cytometry) and DNA fragmentation. The results indicate that hydroxyl radicals induce apoptosis in rat cerebellar neuronal cells, and this induction can be prevented by EGb.

Journal

Neuroscience LettersElsevier

Published: Aug 23, 1996

References

  • Oxygen radicals as key mediators in neurological disease: fact or fiction
    Halliwell, B.
  • Endogenous amino acid release from cultured cerebellar neuronal cells: effect of tetanus toxin on glutamate release
    Van-Vliet, B.J.; Sebben, M.; Dumuis, A.; Gabrion, J.; Bockaert, J.; Pin, J.P.

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