PhTx4, a new class of toxins from Phoneutria nigriventer spider venom, inhibits the glutamate uptake in rat brain synaptosomes

PhTx4, a new class of toxins from Phoneutria nigriventer spider venom, inhibits the glutamate... We report the characterization of a new class of glutamate uptake inhibitors isolated from Phoneutria nigriventer venom. Glutamate transport activity was assayed in rat cerebrocortical synaptosomes by using ( 3 H )- l -glutamate. PhTx4 inhibited glutamate uptake in a dose dependent manner. The IC 50 value obtained was 2.35±0.9 μg/ml which is in the observed range reported for glutamate uptake blockers. Tx4-7, one of PhTx4 toxins, showed the strongest inhibitory activity (50.3±0.69%, n =3). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

PhTx4, a new class of toxins from Phoneutria nigriventer spider venom, inhibits the glutamate uptake in rat brain synaptosomes

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Publisher
Elsevier
Copyright
Copyright © 1999 Elsevier Science B.V.
ISSN
0006-8993
D.O.I.
10.1016/S0006-8993(99)01472-9
Publisher site
See Article on Publisher Site

Abstract

We report the characterization of a new class of glutamate uptake inhibitors isolated from Phoneutria nigriventer venom. Glutamate transport activity was assayed in rat cerebrocortical synaptosomes by using ( 3 H )- l -glutamate. PhTx4 inhibited glutamate uptake in a dose dependent manner. The IC 50 value obtained was 2.35±0.9 μg/ml which is in the observed range reported for glutamate uptake blockers. Tx4-7, one of PhTx4 toxins, showed the strongest inhibitory activity (50.3±0.69%, n =3).

Journal

Brain ResearchElsevier

Published: Jun 12, 1999

References

  • Therapeutic horizons for amyotrophic lateral sclerosis
    Rothstein, J.D.
  • Motor discoordination and increased susceptibility to cerebellar injury in GLAST mutant mice
    Watase, K.; Hashimoto, K.; Kano, M.; Yamada, K.; Watanabe, M.; Inoue, Y.; Okuyama, S.; Sakagawa, T.; Ogawa, S.; Kawashima, N.; Hori, S.; Takimoto, M.; Wada, K.; Tanaka, K.

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