Phospholipid breakdown and choline release under hypoxic conditions: inhibition by bilobalide, a constituent of Ginkgo biloba

Phospholipid breakdown and choline release under hypoxic conditions: inhibition by bilobalide, a... A marked increase of choline release from rat hippocampal slices was observed when the slices were superfused with oxygen-free buffer, indicating hypoxia-induced hydrolysis of choline-containing phospholipids. This increase of choline release was suppressed by bilobalide, an ingredient of Ginkgo biloba , but not by a mixture of ginkgolides. The EC 50 value for bilobalide was 0.38 μ M. In ex vivo experiments, bilobalide also inhibited hypoxia-induced choline release when given p.o. in doses of 2–20 mg/kg 1 h prior to slice preparation. The half-maximum effect was observed with 6 mg/kg bilobalide. A similar effect was noted after p.o. administration of 200 mg/kg EGb 761, a ginkgo extract containing approximately 3% of bilobalide. We conclude that ginkgo extracts can suppress hypoxia-induced membrane breakdown in the brain, and that bilobalide is the active constituent for this effect. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Phospholipid breakdown and choline release under hypoxic conditions: inhibition by bilobalide, a constituent of Ginkgo biloba

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Publisher
Elsevier
Copyright
Copyright © 1997 Elsevier Science B.V.
ISSN
0006-8993
D.O.I.
10.1016/S0006-8993(97)00239-4
Publisher site
See Article on Publisher Site

Abstract

A marked increase of choline release from rat hippocampal slices was observed when the slices were superfused with oxygen-free buffer, indicating hypoxia-induced hydrolysis of choline-containing phospholipids. This increase of choline release was suppressed by bilobalide, an ingredient of Ginkgo biloba , but not by a mixture of ginkgolides. The EC 50 value for bilobalide was 0.38 μ M. In ex vivo experiments, bilobalide also inhibited hypoxia-induced choline release when given p.o. in doses of 2–20 mg/kg 1 h prior to slice preparation. The half-maximum effect was observed with 6 mg/kg bilobalide. A similar effect was noted after p.o. administration of 200 mg/kg EGb 761, a ginkgo extract containing approximately 3% of bilobalide. We conclude that ginkgo extracts can suppress hypoxia-induced membrane breakdown in the brain, and that bilobalide is the active constituent for this effect.

Journal

Brain ResearchElsevier

Published: May 2, 1997

References

  • Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine
    Israel, M.; Lesbats, B.
  • Output, tissue levels, and synthesis of acetylcholine during and after transient forebrain ischemia in the rat
    Kumagae, Y.; Matsui, Y.

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