An elicitor of plant disease resistance, pectinase, was produced by solid state fermentation with Aspergillus niger . Sugar beet pulp was used as carbon source and the wastewater from monosodium glutamate production was used as nitrogen and water source. The composition of the fermentation medium was: 11 ml concentrated wastewater (containing NH 3 –N 38.2 mg/ml), sugar beet pulp 10 g, Na 2 HPO 4 · 12H 2 O 0.2 g, KH 2 PO 4 0.04 g in a 500 ml Erlenmeyer flask. The fermentation temperature was 30 °C and the relative humidity of the air was 75–90%. The maximum production of pectinase was reached after 96 h cultivation. The crude pectinase extracted from the fermented materials could elicit disease resistance in cucumber and tomato seedlings.
Bioresource Technology – Elsevier
Published: Oct 1, 2004
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