PCR-DGGE fingerprinting: novel strategies for detection of microbes in food

PCR-DGGE fingerprinting: novel strategies for detection of microbes in food Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isolated from food, the evaluation of microbial diversity during food fermentation, and microbiological and commercial food quality assessment. Potentials and limitations of this culture-independent approach in food microbiology are indicated and future perspectives are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal Of Microbiological Methods Elsevier

PCR-DGGE fingerprinting: novel strategies for detection of microbes in food

Journal Of Microbiological Methods, Volume 56 (3) – Mar 1, 2004

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Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier B.V.
ISSN
0167-7012
eISSN
1872-8359
D.O.I.
10.1016/j.mimet.2003.11.006
Publisher site
See Article on Publisher Site

Abstract

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isolated from food, the evaluation of microbial diversity during food fermentation, and microbiological and commercial food quality assessment. Potentials and limitations of this culture-independent approach in food microbiology are indicated and future perspectives are discussed.

Journal

Journal Of Microbiological MethodsElsevier

Published: Mar 1, 2004

References

  • Effects of temperature and fertilizer on activity and community structure of soil ammonia oxidizers
    Avrahami, S.; Liesack, W.; Conrad, R.
  • A novel polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) for the identification of Micrococcaceae strains involved in meat fermentations. Its application to naturally fermented Italian sausages
    Cocolin, L.; Manzano, M.; Aggio, D.; Cantoni, C.; Comi, G.
  • An application of PCR-DGGE analysis to profile the yeast populations in raw milk
    Cocolin, L.; Aggio, D.; Manzano, M.; Cantoni, C.; Comi, G.
  • Molecular evaluation of microbial diversity occurring in different types of Mozzarella cheese
    Coppola, S.; Blaiotta, G.; Ercolini, D.; Moschetti, G.
  • Molecular fingerprinting of bacterial populations in groundwater and bottled mineral water
    Dewettinck, T.; Hulsbosch, W.; Van Hege, K.; Top, E.M.; Verstraete, W.
  • Use of denaturing gradient gel electrophoresis for analysis of the stool microbiota of hospitalized patients
    Donskey, C.J.; Hujer, A.M.; Das, S.M.; Pultz, N.J.; Bonomo, R.A.; Rice, L.B.
  • Denaturing gradient gel electrophoretic multilocus sequence typing of Staphylococcus aureus isolates
    Gurtler, V.; Garrie, H.D.; Mayall, B.C.
  • Sequence-determined DNA separations
    Lerman, L.S.; Fisher, S.G.; Hurley, I.; Silverstain, K.; Lumelsky, N.
  • Growth and molecular characterization of dental plaque microcosms
    Mc Bain, A.J.; Bartolo, R.G.; Catrenich, C.E.; Charbonneau, D.; Ledder, R.G.; Gilbert, P.
  • DGGE/TGGE a method for identifying genes from natural ecosystems
    Muyzer, G.
  • The impact grassland management on archaeal community structure in upland pasture rhizosphere soil
    Nicol, G.W.; Glover, L.A.; Prosser, J.I.
  • DNA probe and PCR-specific reaction for Lactobacillus plantarum
    Quere, F.; Deschamps, A.; Urdaci, M.C.

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