Patterning of motor neurons by retinoic acid in the chick embryo hindbrain in vitro

Patterning of motor neurons by retinoic acid in the chick embryo hindbrain in vitro Motor neurons are found throughout the developing chick hindbrain, while somatic motor (SM) neurons develop only in rhombomeres 5 to 8 (r5–8), and in r1. In r2–8 neuroepithelial explants from stage 7–10 embryos cultured in collagen gels, we found that motor neurons were generated throughout r2–8, while SM neuron differentiation was restricted to r5–8, as in vivo. Exposure of such explants to retinoic acid (RA) resulted in SM neuron differentiation throughout r2–8, while inclusion of the mesoderm and endoderm suppressed this effect. In explants with mesoderm/endoderm, RA-dependent SM neuron differentiation in rostral rhombomeres was restored by the application of an inhibitor of the RA-degrading enzyme CYP26. We found that the mesoderm/endoderm (either with or without RA) induced Cyp26 expression in the neuroepithelium in vitro, suggesting that the modulatory effect of CYP26 on RA-dependent patterning might be dependent on local signals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular and Cellular Neuroscience Elsevier

Patterning of motor neurons by retinoic acid in the chick embryo hindbrain in vitro

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Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier Science (USA)
ISSN
1044-7431
D.O.I.
10.1016/S1044-7431(03)00020-4
Publisher site
See Article on Publisher Site

Abstract

Motor neurons are found throughout the developing chick hindbrain, while somatic motor (SM) neurons develop only in rhombomeres 5 to 8 (r5–8), and in r1. In r2–8 neuroepithelial explants from stage 7–10 embryos cultured in collagen gels, we found that motor neurons were generated throughout r2–8, while SM neuron differentiation was restricted to r5–8, as in vivo. Exposure of such explants to retinoic acid (RA) resulted in SM neuron differentiation throughout r2–8, while inclusion of the mesoderm and endoderm suppressed this effect. In explants with mesoderm/endoderm, RA-dependent SM neuron differentiation in rostral rhombomeres was restored by the application of an inhibitor of the RA-degrading enzyme CYP26. We found that the mesoderm/endoderm (either with or without RA) induced Cyp26 expression in the neuroepithelium in vitro, suggesting that the modulatory effect of CYP26 on RA-dependent patterning might be dependent on local signals.

Journal

Molecular and Cellular NeuroscienceElsevier

Published: May 1, 2003

References

  • Retinoid signalling and hindbrain patterning
    Gavalas, A; Krumlauf, R
  • A series of normal stages in the development of the chick embryo
    Hamburger, V; Hamilton, H.L
  • Heads or tails? Retinoic acid will decide
    Maden, M
  • Vitamin A deficient quail embryos have half a hindbrain and other neural defects
    Maden, M; Gale, E; Kostetskii, I; Zile, M
  • The midbrain-hindbrain boundary organizer
    Rhinn, M; Brand, M
  • Complementary domains of retinoic acid production and degradation in the early chick embryo
    Swindell, E.C; Thaller, C; Sockanathan, S; Petkovich, M; Jessell, T.M; Eichele, G
  • Vitamin A deficiency results in the dose-dependent acquisition of anterior character and shortening of the caudal hindbrain of the rat embryo
    White, J.C; Highland, M; Kaiser, M; Clagett-Dame, M

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