Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis... 1 Introduction</h5> Hepatitis E (HE) is a liver disease caused by hepatitis E virus (HEV) infection. The virus is transmitted predominantly via the fecal–oral route. There are approximately 20 million incidents of HE infections every year [1] . HE is usually epidemic in developing countries due to its poor sanitation, but occasionally sporadic in developed countries because of consumption of contaminated food. Generally, HE has a lower mortality through worldwide prevalence in young adults while soars to 20% in pregnant women in some regions [2] . HEV is a small non-enveloped virus with single-stranded RNA genome originally identified in a volunteer study in 1983. Increasingly, HEV isolates have been found worldwide and at least four distinct genotypes but with only one serotype have been determined in epidemiology. Genotypes 1 and 2 circulate in humans only, whereas genotypes 3 and 4 are zoonotic [3,4] . Genotype 1–4 HEVs do not grow well in cell culture, and they share high amino acid sequence identity of the capsid protein encoded by ORF2 gene, which constitutes dominant protective epitopes. Thus, many hepatitis E vaccines are based on the ORF2 capsid protein [5] . For example, in 1990s the VLPs of SAR-55 HEV-derived http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Immunopharmacology Elsevier

Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

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Publisher
Elsevier
Copyright
Copyright © 2014 Elsevier B.V.
ISSN
1567-5769
eISSN
1878-1705
D.O.I.
10.1016/j.intimp.2014.10.032
Publisher site
See Article on Publisher Site

Abstract

1 Introduction</h5> Hepatitis E (HE) is a liver disease caused by hepatitis E virus (HEV) infection. The virus is transmitted predominantly via the fecal–oral route. There are approximately 20 million incidents of HE infections every year [1] . HE is usually epidemic in developing countries due to its poor sanitation, but occasionally sporadic in developed countries because of consumption of contaminated food. Generally, HE has a lower mortality through worldwide prevalence in young adults while soars to 20% in pregnant women in some regions [2] . HEV is a small non-enveloped virus with single-stranded RNA genome originally identified in a volunteer study in 1983. Increasingly, HEV isolates have been found worldwide and at least four distinct genotypes but with only one serotype have been determined in epidemiology. Genotypes 1 and 2 circulate in humans only, whereas genotypes 3 and 4 are zoonotic [3,4] . Genotype 1–4 HEVs do not grow well in cell culture, and they share high amino acid sequence identity of the capsid protein encoded by ORF2 gene, which constitutes dominant protective epitopes. Thus, many hepatitis E vaccines are based on the ORF2 capsid protein [5] . For example, in 1990s the VLPs of SAR-55 HEV-derived

Journal

International ImmunopharmacologyElsevier

Published: Jan 1, 2015

References

  • Antigenic determinants of hepatitis E virus and vaccine-induced immunogenicity and efficacy
    Zhao, Q.; Zhang, J.; Wu, T.; Li, S.W.; Ng, M.H.; Xia, N.S.
  • 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis
    Mierau, I.; Kleerebezem, M.
  • Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China
    Shenyang, G.; Dandan, L.; Chen, F.; Shuliang, W.; Tiezhong, Z.
  • A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability
    Ishiyama, M.; Miyazono, Y.; Sasamoto, K.; Ohkura, Y.; Ueno, K.
  • Expression of carboxymethylcellulase on the surface of Escherichia coli using Pseudomonas syringae ice nucleation protein
    Jung, H.C.; Park, J.H.; Park, S.H.; Lebeault, J.M.; Pan, J.G.

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