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sugars either individually or simultaneously. An increasing popular LC method involves the use of derivatizing reducing sugars like glucose with 1 - phenyl - 3 -methyl-5-pyrazolone (PMP) (Fig 1 ). First proposed ...
for 1 min, the primary reaction products from hemicellulose as well as cellulose solubilization were monomeric sugars . The lack of oligosaccharides can be attributed to the high proton-to-biomass ratio ...
, molecular mass standard. Lane 1 , crude extract; lane 2, active fractions after phenyl sepharose; lane 3 , active fractions after anion exchange; lane 4, active fractions after hydroxyapatite. 1 Open in new ...
Hz, OCH 2 ), 4.19 (q, 2H, J = 7 Hz, COOCH 2 ), 2.51 (t, 2H, J = 7 Hz, CH 2 COO), 2.00 (m, 4H, 2 CH 2 ), 1.32 (t, 3H, J = 7 Hz, CH 3 ), −2.82 (s, 2H, NH). 5-[4-(carboxy- 1 -butyloxy) phenyl ]-10,15,20-tris ...
was for improving PpManR activity towards CLD, the volume of the supernatant and the CLD concentration were reduced to 2 μl and 500 μM, respectively, and the reaction was carried out at ambient temperature for 1 h ...
to nectar (Roy et al., 2017). As such, we first examined starch and soluble sugar accumulation from 3 d (−72 h) to 1 d (−24 h) before secretion (see Fig. 1 , A and B for developmental series) in order ...
reducing condition. The eluate (20 μL) was applied to 12% SDS-PAGE and protein was detected by silver staining using silver stain kit Wako. As controls, 1 % CMC ( 3 mL) alone and AkEG21 ( 1 mg) incubated at 37 ...
between two F and the 3 ′ and penultimate 3 ′ bases (26–28). Computer modeling has hinted that G:H π–π interactions are vital for aligning and binding RNA transcripts to the b-subunit of RNA polymerases (29 ...
substrates compared to buffer only (control) after 24 h incubation (Fig. 5). When wheat straw powder was used as the substrate, there was a significant increase in the release of reducing sugars after 1 -h ...
, a design switch between human galectins had recently been accomplished: Gal- 1 's CRD had been combined with the NT of the chimera-type Gal- 3 to turn homodimeric Gal- 1 into a Gal- 3 -like Gal- 1 variant termed ...
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