Optimization and characterization of nisin-loaded alginate-chitosan nanoparticles with antimicrobial activity in lean beef

Optimization and characterization of nisin-loaded alginate-chitosan nanoparticles with... Nanoencapsulation may improve antimicrobial activity of nisin when applied on food systems such as lean beef. Nisin-loaded nanoparticles were prepared by alginate ionic gelation and further complexation with chitosan. A 3-factor, 3-level Box-Behnken experimental design and response surface methodology were used to optimize the formulation. Nanoparticles had an encapsulation efficiency of 36.1 ± 0.6%, a z-average of 66.4 ± 8.9 nm and a zeta potential of −31.7 ± 2.6 mV. Transmission electron microscopy and atomic force microscopy observations confirmed particle size found by dynamic light scattering. Also, Raman spectroscopy analyses and zeta potential measurements showed ionic interactions between nisin and alginate. In vitro assessment of inhibition of Listeria monocytogenes growth at 4 °C indicated sustained antimicrobial activity of nisin-loaded nanoparticles for 21 days. Furthermore, encapsulated nisin was able to inhibit L. monocytogenes growth in vacuum-sealed, refrigerated beef samples for 10 days when applied at 400 IU/g and for 24 days when applied at 800 IU/g, while free nisin controlled microbial growth for only 4 and 17 days respectively. In sum, we describe the application of experimental design tools to produce nisin-loaded nanoparticles that may be used in the food industry to control microbial growth and hence extend the shelf-life of lean beef. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png LWT - Food Science and Technology Elsevier

Optimization and characterization of nisin-loaded alginate-chitosan nanoparticles with antimicrobial activity in lean beef

Loading next page...
 
/lp/elsevier/optimization-and-characterization-of-nisin-loaded-alginate-chitosan-SuQhy6lXHz
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
0023-6438
D.O.I.
10.1016/j.lwt.2018.01.015
Publisher site
See Article on Publisher Site

Abstract

Nanoencapsulation may improve antimicrobial activity of nisin when applied on food systems such as lean beef. Nisin-loaded nanoparticles were prepared by alginate ionic gelation and further complexation with chitosan. A 3-factor, 3-level Box-Behnken experimental design and response surface methodology were used to optimize the formulation. Nanoparticles had an encapsulation efficiency of 36.1 ± 0.6%, a z-average of 66.4 ± 8.9 nm and a zeta potential of −31.7 ± 2.6 mV. Transmission electron microscopy and atomic force microscopy observations confirmed particle size found by dynamic light scattering. Also, Raman spectroscopy analyses and zeta potential measurements showed ionic interactions between nisin and alginate. In vitro assessment of inhibition of Listeria monocytogenes growth at 4 °C indicated sustained antimicrobial activity of nisin-loaded nanoparticles for 21 days. Furthermore, encapsulated nisin was able to inhibit L. monocytogenes growth in vacuum-sealed, refrigerated beef samples for 10 days when applied at 400 IU/g and for 24 days when applied at 800 IU/g, while free nisin controlled microbial growth for only 4 and 17 days respectively. In sum, we describe the application of experimental design tools to produce nisin-loaded nanoparticles that may be used in the food industry to control microbial growth and hence extend the shelf-life of lean beef.

Journal

LWT - Food Science and TechnologyElsevier

Published: May 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off