Optimising the Siemens Coat-A-Count Radioimmunoassay to Measure Insulin in Equine Plasma

Optimising the Siemens Coat-A-Count Radioimmunoassay to Measure Insulin in Equine Plasma <h5>Introduction</h5> As insulin resistant horses risk laminitis, 1 accurate measurement of plasma insulin concentrations is important. The Siemens Coat-A-Count Insulin Radioimmunoassay (RIA) (Siemens Medical Solutions Diagnostics, Los Angeles, CA) is widely used to quantify insulin in equine plasma. This assay is optimised to detect human insulin, with an anti-human insulin antibody, but there is good reason to anticipate cross-reactivity based on the highly conserved nature of insulin among mammalian species. 2 We evaluated this assay due to concerns that the RIA did not measure equine insulin accurately when high concentrations were determined through dilution.</P><h5>Materials & Methods</h5> The Siemens Coat-A-Count Insulin assay is a solid-phase RIA, wherein 125 I-labelled insulin competes with endogenous insulin for binding sites on an insulin-specific antibody.</P>Samples were equine plasma collected from previous studies in which high and low insulin concentrations had been observed. All samples were collected into lithium heparin tubes, kept on ice, centrifuged at 4 °C within 2 h and stored at -20 °C, until being assayed in duplicate.</P>An internal laboratory quality control (QC) sample of pooled equine plasma was assayed in duplicate at the start, middle and end of each assay, to determine intra-assay variation. Studies of dilutional parallelism and recovery http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Equine Veterinary Science Elsevier

Optimising the Siemens Coat-A-Count Radioimmunoassay to Measure Insulin in Equine Plasma

Loading next page...
 
/lp/elsevier/optimising-the-siemens-coat-a-count-radioimmunoassay-to-measure-gCPdz70lkP
Publisher
Elsevier
Copyright
Copyright © 2009 Elsevier Inc.
ISSN
0737-0806
eISSN
1542-7412
DOI
10.1016/j.jevs.2009.04.110
Publisher site
See Article on Publisher Site

Abstract

<h5>Introduction</h5> As insulin resistant horses risk laminitis, 1 accurate measurement of plasma insulin concentrations is important. The Siemens Coat-A-Count Insulin Radioimmunoassay (RIA) (Siemens Medical Solutions Diagnostics, Los Angeles, CA) is widely used to quantify insulin in equine plasma. This assay is optimised to detect human insulin, with an anti-human insulin antibody, but there is good reason to anticipate cross-reactivity based on the highly conserved nature of insulin among mammalian species. 2 We evaluated this assay due to concerns that the RIA did not measure equine insulin accurately when high concentrations were determined through dilution.</P><h5>Materials & Methods</h5> The Siemens Coat-A-Count Insulin assay is a solid-phase RIA, wherein 125 I-labelled insulin competes with endogenous insulin for binding sites on an insulin-specific antibody.</P>Samples were equine plasma collected from previous studies in which high and low insulin concentrations had been observed. All samples were collected into lithium heparin tubes, kept on ice, centrifuged at 4 °C within 2 h and stored at -20 °C, until being assayed in duplicate.</P>An internal laboratory quality control (QC) sample of pooled equine plasma was assayed in duplicate at the start, middle and end of each assay, to determine intra-assay variation. Studies of dilutional parallelism and recovery

Journal

Journal of Equine Veterinary ScienceElsevier

Published: May 1, 2009

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off