Novel, Not Adenylyl Cyclase-Coupled Cannabinoid Binding Site in Cerebellum of Mice

Novel, Not Adenylyl Cyclase-Coupled Cannabinoid Binding Site in Cerebellum of Mice In this study we report data suggesting the presence of a non-CB1, non-CB2 cannabinoid site in the cerebellum of CB1 −/− mice. We have carried out ( 35 S)GTPγS binding experiments in striata, hippocampi, and cerebella of CB1 −/− and CB1 +/+ mice with Δ 9 -THC, WIN55,212-2, HU-210, SR141716A, and SR144528. In CB1 −/− mice Δ 9 -THC and HU-210 did not stimulate ( 35 S)GTPγS binding. However, WIN55,212-2 was able to stimulate ( 35 S)GTPγS binding in cerebella of CB1 −/− mice. The maximal effect of this stimulation was 31% that of wild type animals. This effect was reversible neither by CB1 nor CB2 receptor antagonists. Similar results were obtained with the endogenous cannabinoid, anandamide. However, adenylyl cyclase was not inhibited by WIN55,212-2 or anandamide in the CB1 −/− animals. In striata and hippocampi of CB1 −/− mice no ( 35 S)GTPγS stimulation curve could be obtained with WIN55,212. Our findings suggest that there is a non-CB1 non-CB2 receptor present in the cerebellum of CB1 −/− mice. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science (USA)
ISSN
0006-291x
D.O.I.
10.1006/bbrc.2002.6635
Publisher site
See Article on Publisher Site

Abstract

In this study we report data suggesting the presence of a non-CB1, non-CB2 cannabinoid site in the cerebellum of CB1 −/− mice. We have carried out ( 35 S)GTPγS binding experiments in striata, hippocampi, and cerebella of CB1 −/− and CB1 +/+ mice with Δ 9 -THC, WIN55,212-2, HU-210, SR141716A, and SR144528. In CB1 −/− mice Δ 9 -THC and HU-210 did not stimulate ( 35 S)GTPγS binding. However, WIN55,212-2 was able to stimulate ( 35 S)GTPγS binding in cerebella of CB1 −/− mice. The maximal effect of this stimulation was 31% that of wild type animals. This effect was reversible neither by CB1 nor CB2 receptor antagonists. Similar results were obtained with the endogenous cannabinoid, anandamide. However, adenylyl cyclase was not inhibited by WIN55,212-2 or anandamide in the CB1 −/− animals. In striata and hippocampi of CB1 −/− mice no ( 35 S)GTPγS stimulation curve could be obtained with WIN55,212. Our findings suggest that there is a non-CB1 non-CB2 receptor present in the cerebellum of CB1 −/− mice.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Mar 22, 2002

References

  • Binding of aminoalkylindoles to noncannabinoid binding sites in NG108-15 cells
    Stark, S.; Pacheco, M.A.; Childers, S.R.
  • Cannabinoid withdrawal is dependent upon PKA activation in the cerebellum
    Tzavara, E.T.; Valjent, E.; Firmo, C.; Mas, M.; Beslot, F.; Defer, N.; Roques, B.P.; Hanoune, J.; Maldonado, R.

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