•Red blood cell lysis artifacts appear in white blood cell scatter profiles.•Avoiding lysis artifacts is attractive for combining cell function and phenotype.•Presented are theory and methods for running and interpreting no lyse no wash assays.•Violet laser 405 nm side scatter separates red blood cells from white blood cells and platelets.•Fluorescence triggering of labeled white blood cells is required for analysis of large cell numbers.
Methods – Elsevier
Published: Feb 1, 2018
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