No lyse no wash flow cytometry for maximizing minimal sample preparation

No lyse no wash flow cytometry for maximizing minimal sample preparation •Red blood cell lysis artifacts appear in white blood cell scatter profiles.•Avoiding lysis artifacts is attractive for combining cell function and phenotype.•Presented are theory and methods for running and interpreting no lyse no wash assays.•Violet laser 405 nm side scatter separates red blood cells from white blood cells and platelets.•Fluorescence triggering of labeled white blood cells is required for analysis of large cell numbers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Methods Elsevier

No lyse no wash flow cytometry for maximizing minimal sample preparation

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
1046-2023
eISSN
1095-9130
D.O.I.
10.1016/j.ymeth.2017.12.012
Publisher site
See Article on Publisher Site

Abstract

•Red blood cell lysis artifacts appear in white blood cell scatter profiles.•Avoiding lysis artifacts is attractive for combining cell function and phenotype.•Presented are theory and methods for running and interpreting no lyse no wash assays.•Violet laser 405 nm side scatter separates red blood cells from white blood cells and platelets.•Fluorescence triggering of labeled white blood cells is required for analysis of large cell numbers.

Journal

MethodsElsevier

Published: Feb 1, 2018

References

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