NMR evidence for different conformations of the bioactive region of rat CCK-8 and CCK-58

NMR evidence for different conformations of the bioactive region of rat CCK-8 and CCK-58 Sulfated CCK-58 and CCK-8 have identical bioactive C-terminal primary sequences but distinct C-terminal solution structures and different bioactivities. To examine structural differences in greater detail, rat CCK-58 and -8 were synthesized with isotopic enrichment of C-terminal residues with 15 N at α-amino nitrogens. Proton and nitrogen chemical shift assignments of peptide solutions were obtained by homo- and heteronuclear NMR methods. These data show that the tertiary structure ensembles of C-terminal CCK-8 and CCK-58 differ significantly. Thus, distinct solution conformations may explain differences in CCK A and CCK B receptor interactions of large and small molecular forms of CCK. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

NMR evidence for different conformations of the bioactive region of rat CCK-8 and CCK-58

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science (USA)
ISSN
0006-291x
DOI
10.1016/S0006-291X(02)00309-1
pmid
12051761
Publisher site
See Article on Publisher Site

Abstract

Sulfated CCK-58 and CCK-8 have identical bioactive C-terminal primary sequences but distinct C-terminal solution structures and different bioactivities. To examine structural differences in greater detail, rat CCK-58 and -8 were synthesized with isotopic enrichment of C-terminal residues with 15 N at α-amino nitrogens. Proton and nitrogen chemical shift assignments of peptide solutions were obtained by homo- and heteronuclear NMR methods. These data show that the tertiary structure ensembles of C-terminal CCK-8 and CCK-58 differ significantly. Thus, distinct solution conformations may explain differences in CCK A and CCK B receptor interactions of large and small molecular forms of CCK.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: May 10, 2002

References

  • Biopolymers
    Fournie-Zaluski, M.C; Durieux, C; Lux, B; Belleney, J; Pham, P; Gerard, D; Roques, B.P
  • Biochemistry
    Koshland, D.E.J; Nemethy, G; Filmer, D
  • Biochemistry
    Keire, D.A; Kobayashi, M; Solomon, T.E; Reeve, J.R

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