In this study nickel nanoclusters (NiNCs), was promised as novel and economic electrochemiluminescence (ECL) emitter for highly sensitive and selective determination of creatinine in the presence of molecularly imprinted polymer (MIP). The uniform magnetic graphene oxide (GO-Fe3O4) MIP film was established on the surface of ITO electrode and Ni NCs-embedded in MIP, showed a strong anodic ECL emission using tri-n-propylamine (TPrA) as coreactant. During the ECL process, TPrA was oxidized, and Ni NCs got the energy to generate excited state Ni NCs* for light emission. When the imprinted cavities were occupied by creatinine, the ECL emission of Ni NCs on the MIP-modified electrode surface was efficiently quenched. Under optimal conditions, the proposed sensor demonstrated ultrasensitive and accurate analytical performance toward creatinine detection with a linear range from 5 nM to 1 mM and detection limit of 0.5 nM (S/N = 3). The biosensor showed good specificity for creatinine determination compared to other compounds that having the chemical structure analogue or close to the template creatinine, when the concentration ratio of interference to creatinine was more than 100 times. Furthermore, the biosensor was successfully applied to determination of creatinine in human serum and urine samples with satisfactory results. So, this assay for creatinine detection possesses high sensitivity, good selectivity, excellent reproducibility and stability. We expect the combination of molecular imprinting system with ECL assay in the presence of Ni NCs as a new type of superior luminophore candidate can be developed for design of ultrasensitive sensors, biosensors and other measuring devices.
Biosensors and Bioelectronics – Elsevier
Published: Jun 1, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera