Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G

Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of... Tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) are important mediators of intracerebral haemorrhage (ICH) inflammatory response. Lazaroids, established antioxidants and neuroprotectants, have been studied in several brain pathologies. The present study was designed to investigate: a) TNF-α and IL-1β changes, in neurons and b) U-74389G effects, 4 and 24h after haematoma induction in a porcine model of intracerebral haemorrhage.In twenty male landrace pigs (swines) aged 135–150 days old, autologous whole blood was injected around the right basal ganglia territory; in ten of the pigs the lazaroid compound U-74389G was administered. Brain TNF-α and IL-1β immunopositive neurons were determined by immunoarray techniques at 4 and 24h timepoints.After the haematoma induction the number of TNF-α immunopositive neurons ipsilateral to the haematoma was significantly higher compared to the contralateral site at 4h (p<0.0005), while U-74389G significantly reduced the number of TNF-α immunopositive neurons, ipsilateral to the haematoma, at 4h (p=0.002); at 24h, TNF-α immunopositive neurons were found significantly lower in the control group ipsilateral to the haematoma in comparison to 4h timepoint(p<0.0005).The number of IL-1β immunopositive neurons at 4h after the hematoma induction was significantly higher ipsilateral to the haematoma site (p<0.0005). U-74389G had no statistical significant effect.TNF-α and IL-1β, increase in neurons, 4h after the haematoma induction, ipsilateral to the haematoma site. The administration of the antioxidant compound U-74389G, results in early (at 4h) decrease of TNF-α immunopositive neurons but shows no statistical significant effect to IL-1β immunopossitive neurons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G

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Publisher
Elsevier
Copyright
Copyright © 2015 Elsevier B.V.
ISSN
0006-8993
D.O.I.
10.1016/j.brainres.2015.04.034
Publisher site
See Article on Publisher Site

Abstract

Tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) are important mediators of intracerebral haemorrhage (ICH) inflammatory response. Lazaroids, established antioxidants and neuroprotectants, have been studied in several brain pathologies. The present study was designed to investigate: a) TNF-α and IL-1β changes, in neurons and b) U-74389G effects, 4 and 24h after haematoma induction in a porcine model of intracerebral haemorrhage.In twenty male landrace pigs (swines) aged 135–150 days old, autologous whole blood was injected around the right basal ganglia territory; in ten of the pigs the lazaroid compound U-74389G was administered. Brain TNF-α and IL-1β immunopositive neurons were determined by immunoarray techniques at 4 and 24h timepoints.After the haematoma induction the number of TNF-α immunopositive neurons ipsilateral to the haematoma was significantly higher compared to the contralateral site at 4h (p<0.0005), while U-74389G significantly reduced the number of TNF-α immunopositive neurons, ipsilateral to the haematoma, at 4h (p=0.002); at 24h, TNF-α immunopositive neurons were found significantly lower in the control group ipsilateral to the haematoma in comparison to 4h timepoint(p<0.0005).The number of IL-1β immunopositive neurons at 4h after the hematoma induction was significantly higher ipsilateral to the haematoma site (p<0.0005). U-74389G had no statistical significant effect.TNF-α and IL-1β, increase in neurons, 4h after the haematoma induction, ipsilateral to the haematoma site. The administration of the antioxidant compound U-74389G, results in early (at 4h) decrease of TNF-α immunopositive neurons but shows no statistical significant effect to IL-1β immunopossitive neurons.

Journal

Brain ResearchElsevier

Published: Jul 30, 2015

References

  • Inhibition of tumour necrosis factor and reversal of endotoxin-induced shock by U-83836E, a second generation׳ lazaroid in rats
    Altavilla, D.; Squadrito, F.; Serrano, M.; Campo, G.M.; Squadrito, G.; Arlotta, M.; Urna, G.; Sardella, A.; Saitta, A.; Caputi, A.P.
  • Activation of acetylcholinesterase after U-74389G administration in a porcine model of intracerebral hemorrhage
    Bimpis, A.; Papalois, A.; Tsakiris, S.; Zarros, A.; Kalafatakis, K.; Botis, J.; Stolakis, V.; Zissis, K.M.; Liapi, C.
  • The lazaroid U74389G protects normal brain from stereotactic radiosurgery-induced radiation injury
    Buatti, J.M.; Friedman, W.A.; Theele, D.P.; Bova, F.J.; Mendenhall, W.M.
  • Recent research on changes in genomic regulation and protein expression in intracerebral haemorrhage
    Cannon, J.R.; Xi, G.; Keep, R.F.
  • Acute inflammatory reaction following experimental intracerebral hemorrhage in rat
    Gong, C.; Hoff, J.T.; Keep, R.F.
  • TNF-alpha receptor antagonist, R-7050, improves neurological outcomes following intracerebral hemorrhage in mice
    King, M.D.; Alleyne, C.H.; Dhandapani, K.M.
  • The duality of the inflammatory response to traumatic brain injury
    Lenzlinger, P.M.; Morganti-Kossmann, M.C.; Laurer, H.L.; McIntosh, T.K.
  • Antioxidant compounds protect dopamine neurons from death due to oxidative stress in vitro
    Stull, N.D.; Polan, D.P.; Iacovitti, L.
  • Effects of selective hypothermia on blood–brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats
    Sun, H.; Tang, Y.; Guan, X.; Li, L.; Wang, D.
  • Inflammation after intracerebral hemorrhage
    Wang, J.; Dore ́, S.

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